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Use of taspine for preparing medicament for inhibiting tumor angiogenesis

A technology for tumor angiogenesis and taspine, which can be used in anti-tumor drugs, drug combinations, etc., and can solve the problem of undiscovered taspine

Inactive Publication Date: 2007-08-08
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Taspinine is a kind of aporfiline alkaloid. Its preparation method and application in tumor treatment have applied for invention patents. However, according to the applicant's data retrieval, it has not been found that taspinine is used to prepare tumor-inhibiting blood vessels. Generating Drug Reports

Method used

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  • Use of taspine for preparing medicament for inhibiting tumor angiogenesis
  • Use of taspine for preparing medicament for inhibiting tumor angiogenesis
  • Use of taspine for preparing medicament for inhibiting tumor angiogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0020] 1. Establishment of xenograft model of chick embryo allantoic membrane tumor

[0021] 1. Instruments and materials

[0022] Dissecting microscope (Shanghai No. 6 Optical Instrument Factory); CO 2 Constant temperature incubator (SANYOMCO-17AC, Japan); ultra-clean bench (SW-CJ-1F, Suzhou Antai Air Technology Co., Ltd.).

[0023] 2. Experimental method

[0024] 2.1 CAM model preparation

[0025] The fertilized eggs were washed twice with warm water, soaked in 1:1000 benzalkonium bromide solution for 5 minutes, and then incubated in an incubator with a temperature of 37.8±0.2°C and a humidity of 40% to 60%. On the 6th day of incubation, disinfect the 2×3cm area on the surface of the eggshell with alcohol, make a dent on the surface of the eggshell with a dental drill, remove the outer shell and shell membrane of the egg, and gently tear off the inner shell membrane. The CAM sinks, forming a false air chamber.

[0026] Seal the false air chamber with sterile parafilm t...

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Abstract

The invention relates to a method for using tasepi alkali to prepare the drug that restrains the growth of tumor vessel, which provides the method for building chicken blast allantois membrane non-small-cell lung cancer A549 tumor transplant cancer model. The invention uses said test model, ELISA, immunity protein label, and Real Time RCR test method to research the effects of tasepi alkali on the CAM vessel structure, the growth of tumor, the shape change and the fresh vessel distribution of tumor, the protein expression of VEGF and bFGF, the gene expressions of VEGF acceptors as flt-1 and flk-1 / KDR, the protein expressions of AKT and Erk1 / 2, and the phosphorylation level, to prove the function of tasepi alkali for restraining the growth of tumor vessel.

Description

technical field [0001] The invention belongs to the field of compound pharmacological action and molecular biology technology, and specifically relates to the application of taspinine in the preparation of drugs for inhibiting tumor angiogenesis. Background technique [0002] Angiogenesis refers to the regeneration of new blood vessels on the original vascular bed, which is a complex multifactorial process. Angiogenesis is essential in the occurrence and metastasis of tumors. Studies have found that a series of oncogenes, tumor suppressor genes and growth factors play an important role in regulating tumor angiogenesis. The initiation or conversion of tumor angiogenesis phenotype depends on the balance and coordination of angiogenesis factors and their inhibitors. The complex proteolytic balance system completes the migration, invasion and proliferation of endothelial cells, and finally forms tumor neovascularization. . Blocking angiogenesis is a new means and way to treat ...

Claims

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Application Information

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IPC IPC(8): A61K31/365A61P35/00
Inventor 贺浪冲张彦民罗文娟赵静王嗣岑孟梁李西玲李旭张三奇
Owner XI AN JIAOTONG UNIV
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