Method for preparing peroxidase of radish
A technology of peroxidase and radish, applied in the biological field, can solve the problems of high cost, restricted development and utilization, and complicated extraction process.
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[0005] The specific embodiment of the present invention is as follows: take 1 ton of fresh radish, crush it into a paste, place it in a high-speed refrigerated centrifuge to separate the radish juice, and then extract the radish residue with buffer solution, the volume increases by 3-5 times, but the obtained The peroxidase is only 1 / 10 of the original juice. When the obtained radish juice is added to ammonium sulfate powder step by step to make the saturation reach 30-50%, let it stand for 10-18 hours. After discarding the sediment, take Supernatant, continue to add ammonium sulfate powder to the supernatant to make the saturation reach 75-90%, let it stand for 10-18 hours, then discard the supernatant, dilute the precipitate with water, filter, and filter the filtered filtrate Use 20×17cm, 100kD PES ultrafiltration membrane for ultrafiltration and desalination, the permeate that passes through the membrane can be further recycled, and the concentrated solution that does not p...
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