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Chitin oligose preparing process

A chitosan oligosaccharide and chitin technology, applied in the field of chitin oligosaccharide, can solve problems such as application limitation and incapability of mass production, and achieve the effects of realizing industrialized production, reducing pollution and simple process route

Inactive Publication Date: 2009-08-12
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chitosanase and chitinase are not yet mass-produced, so applications are limited
The method of preparing chitooligosaccharides by enzymatic hydrolysis with specific hydrolase has not been reported so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] 1. Strain cultivation and preparation of high-activity enzyme solution

[0059] Take a ring of Aeromonas caviae Q2 strain from the inclined plane and insert it into a 500ml shake flask with 100ml culture solution, shake it at 35°C for 24 hours to obtain the shake flask seed solution, and the shake flask seed medium contains peptone 1%, 5% yeast extract, 5% corn steep liquor, and 1% NaCl were inserted into cultures containing 1% glycerol, 5% yeast extract, 5% corn steep liquor, 1% NaCl and 7% bean cake powder at a 5% inoculum size. Fermented at 35°C for 24 hours in a 10L seed fermenter to obtain a seed culture solution. Add medium solution to a 100L fermenter, which contains 1% glycerin, 2.5% yeast extract, 2.5% corn steep liquor, 1% NaCl, 5% bean cake powder, sterilize at 121°C for 20min, add 10L of seed culture solution, and Cultivate for 24 hours at 35°C, with a rotation speed of 100-300r / min and an air flow of 100l / min, to obtain a fermentation broth containing chit...

Embodiment 2

[0071] 1. Strain cultivation and preparation of high-activity enzyme solution

[0072] Take a ring of Aeromonas caviae Q2 strain from the inclined plane and insert it into a 500ml shake flask with 100ml culture solution, shake it at 35°C for 24 hours to obtain the shake flask seed solution, and the shake flask seed medium contains peptone 1%, 5% yeast extract, 5% corn steep liquor, and 1% NaCl were inserted into cultures containing 1% glycerol, 5% yeast extract, 5% corn steep liquor, 1% NaCl and 7% bean cake powder at a 5% inoculum size. Fermented at 35°C for 24 hours in a 10L seed fermenter to obtain a seed culture solution. Add medium solution to a 100L fermenter, which contains 1.5% glycerin, 0.5% yeast extract, 1% corn steep liquor, 0.5% NaCl, 10% bean cake powder, sterilize at 121°C for 20min, add 10L of seed culture solution, and Cultivate for 40 hours at 25°C, 300r / min, and with an air flow of 5l / min to obtain a fermentation broth containing chitinase, centrifuge in a ...

Embodiment 3

[0086] 1. Strain cultivation and preparation of high-activity enzyme solution

[0087] Take a ring of Aeromonas caviaeQ2 strain from the inclined plane and insert it into a 500ml shake flask with 100ml culture solution, and vibrate at 37°C for 20 hours to obtain the shake flask seed solution, and the shake flask seed medium contains peptone 1%, 5% yeast extract, 5% corn steep liquor, and 1% NaCl were inserted into a culture containing 4% glycerol, 5% yeast extract, 3% corn steep liquor, 1% NaCl and 5% bean cake powder at a 5% inoculum size. Fermented at 37°C for 20 hours in a 10L seed fermenter to obtain seed culture solution. Add medium solution to a 100L fermenter, which contains 4% glycerin, 3% yeast extract, 2% corn steep liquor, 0.5% NaCl, 3% bean cake powder, sterilize at 121°C for 20min, add 10L of seed culture solution, and Under the conditions of 37°C, 200r / min, and ventilation rate of 150l / min, cultivate for 20 hours to obtain a fermentation broth containing chitina...

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PUM

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Abstract

The invention relates to a chitooligosaccharide preparation method, relating to a chitooligosaccharide, in particular to a method for preparing biologically active chitin oligosaccharides by degrading chitin colloids by fermenting an enzymatic method. Provide a simple process route, which can effectively inhibit the further degradation of active chitosan oligosaccharides under the action of enzymes. The degrading enzyme can be reused, and the efficiency of enzyme use is high. Products with different degrees of polymerization can be obtained according to needs, with less pollution and high separation efficiency. , a chitosoligosaccharide preparation method capable of large-scale industrial production. Centrifuge and separate the fermentation broth obtained from the culture of Aeromonas caviae, obtain the supernatant of the fermentation broth, and separate the enzyme solution; then prepare chitin colloid; in the enzyme reaction tank, suspend the chitin colloid in the buffer solution , prepared into a chitin colloid solution, aseptically transporting the chitin enzyme solution to the chitin colloid solution in a sterilized enzyme reaction tank to obtain a chitin oligosaccharide enzymatic hydrolysis solution; The three and four stages of membrane separation spray-dry the intercepted concentrated solution to obtain the active chitosan oligosaccharide product.

Description

technical field [0001] The invention relates to a chitosan oligosaccharide, in particular to a method for preparing biologically active chitosan oligosaccharides by degrading chitin colloids by fermenting and producing enzymes. Background technique [0002] Chitin is a linear polymer formed by connecting N-acetyl-2-deoxy-D glucose (GlcNAc) and a small amount of 2-amino and 2-deoxyglucose with β(1,4) glycosidic bonds. Chitin is a good substrate for chitinase, second only to cellulose in natural polymers. Because it is insoluble in common solvents, its application is limited. [0003] Because in the existing literature, the exact reference components of chitin, chitosan, chitooligosaccharides and chitooligosaccharides etc. are different, now the definitions of each noun used in this patent application are as follows: [0004] Chitin: also known as chitin, is a polymer of 2-acetylamino-2 deoxy-D-glucose linked by β-1,4 glycosidic bonds. [0005] Chitosan: chitosan obtained b...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08C07H3/06C12P19/04
Inventor 乔兴忠李永娴王风平肖湘
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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