Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing HPLC standard fingerprint pattern of dog-ridge medicinal materials and quality identification

A standard fingerprint and fingerprint technology, which is applied in the field of constructing the HPLC standard fingerprint of the dog spine medicinal material and identifying the quality of the medicinal material, achieving the effects of good stability, enhanced identification, and easy grasp

Inactive Publication Date: 2009-07-15
MASSON GROUP
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Until today, there is no report on the method of using reversed-phase high-performance liquid chromatography to detect multiple components of dog ridge at one time and establish fingerprints

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for constructing HPLC standard fingerprint pattern of dog-ridge medicinal materials and quality identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Construction of dog ridge HPLC standard fingerprint

[0029] 1. Instruments and reagents

[0030] 1.1. Instrument

[0031] Agilent1100 liquid chromatograph, DAD detector; Shanghai Brain SB5200 ultrasonic cleaner.

[0032] 1.2. Test drug

[0033] Methanol, analytically pure; acetic acid, analytically pure; acetonitrile, chromatographically pure, produced by Dima Company; ultrapure water, Wahaha purified water; 10 batches of medicinal materials produced in Guangxi.

[0034] The above-mentioned dog spine medicinal materials have been tested to meet the corresponding national standard requirements in the Pharmacopoeia.

[0035] 2. Chromatographic conditions

[0036] The detection wavelength is 350nm; Dima C18 column (200×4.6mm, 5μm); the column temperature is 30°C; the flow rate is 1ml / min; the mobile phase is acetonitrile and 1% acetic acid solution, gradient elution, and the elution ratio is:

[0037] T(min) CH3CN(%) 1%CH3COOH(%)

[0038] 0 5 95

[0039] 20 5 95...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the method of constructing the HPLC standard fingerprint of the medicinal material of dog ridge and identifying the quality of the medicinal material, which is used for the quality identification of the medicinal material of dog ridge. It discloses that the powder of the test product is ultrasonically extracted in 20ml80% methanol solution for 60 minutes, and then filtered to obtain the test product solution. HPLC conditions: chromatographic column Dima C18 column (200×4.6mm, 5μm), column temperature 30°C; mobile phase acetonitrile-1% acetic acid solution, gradient elution, flow rate 1ml / min; detection wavelength 350nm. Take 10 batches of Guangxi-produced dog spine medicinal materials for determination, find common peaks, and establish standard fingerprints by software approximation. There are 8 main common characteristic peaks, ranging from G1 to G8, with retention times of 7.4min, 17.3min, and 21.3min, respectively. 25.5min, 29.8min, 33.0min, 39.3min, 41.0min; then compare the fingerprints determined by the medicinal materials to be tested with the standard fingerprints, and the medicinal materials with a similarity greater than 0.950 are used in production.

Description

technical field [0001] The invention relates to a method for constructing an HPLC standard fingerprint of a dog spine medicinal material and a method for identifying the quality of the medicinal material by using the standard fingerprint. Background technique [0002] Dog ridge medicinal materials are planted in various southern provinces, and the quality varies due to differences in varieties, origins, and harvesting periods. Therefore, the quality, efficacy, and stability of its finished products are difficult to guarantee. The key part of the quality control of the dog ridge in the current national pharmacopoeia standard is the microscopic identification of the cross-section, and the thin-layer identification of protocatechualdehyde and protocatechuic acid. These authenticity identification and quality evaluation for this medicinal material are one-sided. Cross-section microscopic identification can not evaluate the quality of medicinal materials, but can only be used to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/26G01N30/86
Inventor 侯峰刘芳华文俊莫启武程朝晖王宝东王鸿昶刘汉槎
Owner MASSON GROUP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com