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Method for constructing HPLC standard fingerprint pattern of dog-ridge medicinal materials and quality identification

A standard fingerprint and fingerprint technology, which is applied in the field of constructing the HPLC standard fingerprint of the dog spine medicinal material and identifying the quality of the medicinal material, to achieve the effects of easy mastery, enhanced identification, and good stability

Inactive Publication Date: 2007-08-08
MASSON GROUP
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AI Technical Summary

Problems solved by technology

[0005] Until today, there is no report on the method of using reversed-phase high-performance liquid chromatography to detect multiple components of dog ridge at one time and establish fingerprints

Method used

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  • Method for constructing HPLC standard fingerprint pattern of dog-ridge medicinal materials and quality identification

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0028] 1. Construction of dog ridge HPLC standard fingerprint

[0029] 1. Instruments and reagents

[0030] 1.1. Instrument

[0031] Agilent 1100 liquid chromatograph, DAD detector; Shanghai Brain SB5200 ultrasonic cleaner.

[0032] 1.2. Test drug

[0033] Methanol, analytically pure; acetic acid, analytically pure; acetonitrile, chromatographically pure, produced by Dima Company; ultrapure water, Wahaha purified water; 10 batches of medicinal materials produced in Guangxi.

[0034] The above-mentioned dog spine medicinal materials have been tested to meet the corresponding national standard requirements in the Pharmacopoeia.

[0035] 2. Chromatographic conditions

[0036] The detection wavelength is 350nm; Dima C18 column (200×4.6mm, 5μm); the column temperature is 30°C; the flow rate is 1ml / min; the mobile phase is acetonitrile and 1% acetic acid solution, gradient elution, and the elution ratio is:

[0037] T(min) CH3CN(%) 1%CH3COOH(%)

[0038] 0 5 95

[0039] 20...

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Abstract

The invention relates to building barometz medicine HPLC standard fingerprint pattern and identification medicine quality method, to be used in the barometz medicine quality identification, and it discloses that after the trial materials powder ultrasonic extracting 60 minutes in 20ml80% methanol solution, to filter the trial materials solution. HPLC conditions: chromatogram column Dumas C18 column (200X4.6mm, 5mum), column temperature 30deg.C; mobile phase acetonitrile-1% acetic acid solution, gradient elution, flow rate 1ml / min; detection wavelength 350nm. Taking 10 groups Guangxi barometz medicines to determine, to find common characterized peak, with software plausible to establish a standard fingerprint pattern, there are totally eight common characterized peaks, G1 to G8 peaks, retention time being 7.4min, 17.3min, 21.3min, 25.5min, 29.8min, 33.0min, 39.3min, 41.0min. And then, compare the fingerprint pattern determined by the trial medicine and the standard fingerprint pattern, the medicine with similarity more than 0.950 is applied in the product.

Description

technical field [0001] The invention relates to a method for constructing an HPLC standard fingerprint of a dog spine medicinal material and a method for identifying the quality of the medicinal material by using the standard fingerprint. Background technique [0002] Dog ridge medicinal materials are planted in various southern provinces, and the quality varies due to differences in varieties, origins, and harvesting periods. Therefore, the quality, efficacy, and stability of its finished products are difficult to guarantee. The key part of the quality control of the dog ridge in the current national pharmacopoeia standard is the microscopic identification of the cross-section, and the thin-layer identification of protocatechualdehyde and protocatechuic acid. These authenticity identification and quality evaluation for this medicinal material are one-sided. Cross-section microscopic identification can not evaluate the quality of medicinal materials, but can only be used to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/06G01N30/26G01N30/86
Inventor 侯峰刘芳华文俊莫启武程朝晖王宝东王鸿昶刘汉槎
Owner MASSON GROUP
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