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Method for constructing HPLC standard fingerprint pattern of semen cuscutae medicinal materials and quality identification

A standard fingerprint and fingerprint technology are applied in the field of constructing the HPLC standard fingerprint of Cuscuta chinensis medicinal materials and identifying the quality of medicinal materials, which can solve the problems such as unreported, and achieve the effects of enhanced identification, easy to grasp, and high precision.

Inactive Publication Date: 2007-08-08
MASSON GROUP
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AI Technical Summary

Problems solved by technology

[0004] At present, some scholars have studied the method of detecting three flavonoids in Cuscuta by HPLC, but there is no report on the method of detecting multiple components of Cuscuta at one time and establishing a fingerprint by reversed-phase high-performance liquid chromatography

Method used

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  • Method for constructing HPLC standard fingerprint pattern of semen cuscutae medicinal materials and quality identification

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0027] 1. Construction of the standard fingerprint of Cuscuta HPLC

[0028] 1. Instruments and reagents

[0029] 1.1, instrument

[0030] Agilent 1100 liquid chromatograph, DAD detector; Shanghai Bineng SB5200 ultrasonic cleaner.

[0031] 1.2 Test drug

[0032] Methanol, analytically pure; Acetic acid, analytically pure; Acetonitrile, chromatographically pure, produced by Dima; Ultrapure water, Wahaha purified water; 10 batches of Cuscuta medicinal materials produced in Inner Mongolia.

[0033] The above-mentioned Cuscuta seeds have been tested to meet the requirements of the corresponding national standards in the Pharmacopoeia.

[0034] 2. Chromatographic conditions

[0035] Detection wavelength 350nm; Dima C18 column (200×4.6mm, 5μm); column temperature 30℃; flow rate 1ml / min; mobile phase is acetonitrile, 1% acetic acid solution, gradient elution, the elution ratio is:

[0036] T(min) CH3CN(%) 1%CH3COOH(%)

[0037] 0 5 95

[0038] 20 5 95

[0039] 80 45 55

[0040] 90 5 95

[...

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Abstract

The invention relates to construction dodder medicines HPLC standard fingerprint pattern and identification medicine quality method, used for the dodder medicine quality identification, and it discloses the powder of the trial materials ultrasonically extracting 60mins in the 20ml80% methanol solution, and filtering to obtain the trial materials solution; HPLC conditions: chromatogram column Dumas C18 column (200X4.6 mm, 5mum), column temperature 30deg.C; mobile phase acetonitrile-1% acetic acid solution, gradient elution, flow rate 1ml / min; detection wavelength 350nm. Taking 10 groups Inner Mongolia dodder medicines to determine, to find common characterized peak, with software plausible to establish a standard fingerprint pattern, there are totally six common characterized peaks, T1 to T6 peaks, retention time being 31.8min, 44.3min, 46.2min, 46.7 min, 50.1min, 68.4min; and then, compare the fingerprint pattern determined by the trial medicine and the standard fingerprint pattern, the medicine with similarity more than 0.900 is applied in the product.

Description

Technical field [0001] The invention relates to a method for constructing a HPLC standard fingerprint of dodder seed medicinal materials, and a method for identifying the quality of the medicinal materials by using the standard fingerprint pattern. Background technique [0002] Cuscuta seeds are cultivated in many provinces in my country, and the quality is uneven due to different varieties, origins, and harvesting periods. Therefore, the finished products, the quality, efficacy and stability of the products are difficult to guarantee. The key part of the quality control of dodder seeds in the current national pharmacopoeia standards is microscopic identification. These are obviously far from enough for the authenticity identification and quality evaluation of this medicinal material. At present, there are many studies on the chemical components of dodder seeds, and it is reported that they contain flavonoids, sterols and other types of ingredients. However, the active ingredient...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/26G01N30/86
Inventor 侯峰刘芳华文俊莫启武程朝晖王宝东王鸿昶刘汉槎
Owner MASSON GROUP
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