Method for detecting bacterial AmpC enzyme using paper disk containing 3-amino phenol borate

Abstract

This invention discloses a method for testing bacteria AmpC enzyme with paper containing 3-amino-phenol-boric acid. It prepares APB solution first and adds APB on the CAZ, CTX and FOX paper without APB to apply APB paper reinforcement method to carry out paper method drug hypersensitive test to test the AmpC enzyme generated by bacteria. APB can enlarge the microbiostatic ring of CAZ, CTX or FOX paper to the generated AmpC enzyme bacteria so as to judge the existence of AmpC enzyme by testing the change of the diameter of the ring to avoid missed test brought with using the single base material to increase the positive rate.

Description

technical field [0001] The invention belongs to the technical scope of biological bacteria detection, in particular to a method for detecting bacterial AmpC enzyme with a paper sheet containing 3-aminophenol boric acid (APB). Background technique [0002] AmpCβ-lactamases, which belong to the class C enzymes classified by Ambler (hereinafter referred to as AmpC enzymes), are produced by some bacteria such as Klebsiella pneumoniae and Escherichia coli during the growth process and can hydrolyze third-generation cephalosporin antibiotics (such as ceftazidime) CAZ, cefotaxime CTX, ceftriaxone CRO), cephamycin (such as cefoxitin FOX), monoamine antibiotics (such as aztreonam AZT) enzymes. Bacteria that produce AmpC enzyme are resistant to the above antibiotics, and when these bacteria cause infections such as sepsis, pneumonia, pyelonephritis or wound infection, the use of the above antibiotics will make the treatment fail. Therefore, the correct detection of bacteria producing...

AI Technical Summary

Problems solved by technology

But so far, there is no simple and effective method for routine detection of AmpC-producing bacteria in clinical laboratories

Three-dimensional assay to detect AmpC enzyme (Coudron PE, Moland ES, Thomson KS. Occurrence and detection of AmpC beta-lactamases among Escherichia coli, Klebsiella pneumoniae, and proteus mirabilis isolates at a veterans medical center. J ClinMicrobiol, 2000, 38: 1791-1796) , it is necessary to prepare the crude extract of the enzyme and dig grooves on the agar plate, the operation is cumbersome, time-consuming, and requires special equipment such as ultrasonic breaker or repeated freezing and thawing conditions

The latest method for detecting AmpC enzyme with Tris-EDTA introduced abroad (Black JA, Moland ES, and Thomson KS. AmpCdisk test for detection of plasmamid-mediated AmpC β-lactamases in Enterobacteriaceae lacking chromosome AmpC β-lactamases. J ClinMicrobiol, 2005, 43: 3110-3113) Although it has the characteristics of simple operation and low cost, it needs to prepare paper containing Tris-EDTA and the bacteria to be tested, which increases the operation steps, and the interpretation of the results of some bacteria requires certain experience