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Pesticide sevin artificial antigen and antibody and preparation method and application thereof

A technology of artificial antigen and carbaryl, applied in the direction of carrier binding antigen/hapten components, antibodies, measuring devices, etc.

Active Publication Date: 2008-06-04
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The artificial antigen and specific antibody of carbaryl and the establishment of immunoassay method based on it have not been reported yet

Method used

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  • Pesticide sevin artificial antigen and antibody and preparation method and application thereof
  • Pesticide sevin artificial antigen and antibody and preparation method and application thereof
  • Pesticide sevin artificial antigen and antibody and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] 1. Hapten Synthesis

[0067] In the present invention, the structure of 1-naphthyl-N-carbamate (b) is selected as the basic structure of the hapten, and its molecular weight MW is 300. The design synthesized:

[0068] 1) Hapten 41-(5-carboxypentyl)-3-(1-naphthyl)urea

[0069] Mix 1.7g (0.01mol) of 1-naphthyl isocyanic acid and 0.1g (1.30mol) of 6-aminocaproic acid in 100mL of tetrahydrofuran (THF), stir for 12 hours at 22°C, and filter to obtain 2.0 g of the hapten as a solid; recrystallized in acetonitrile and evaporation of the solvent gave 0.9 g of pure compound in an overall yield of 96%.

[0070] 2) Hapten 3 (N-(2-naphthoyl)-6-aminocaproic acid)

[0071] 3.8g (0.02mol) of 2-naphthoyl chloride was dissolved in 20ml of 1,4-dioxane, and in an ice bath, this material was added dropwise to 100ml containing 2.6g (0.02mol) of 6- Aminocaproic acid and 8.0 g (0.30 mol) of potassium hydroxide were mixed in an aqueous solution. The mixture was stirred at 25°C for 4 hours...

Embodiment 2

[0114] During the immunoassay, extract the sample using the methanol extraction method ② that is: use 10g of crushed barley, wheat and sorghum to be tested, respectively, soak in 50ml of methanol and shake for 2 minutes, let it stand, and take the solution to obtain the sample to be tested . The resulting soaking solution is the sample to be tested. Others are with embodiment 1.

Embodiment 3

[0116] During the immunoassay, extract the samples using the methanol extraction method ③ that is: use 10g of crushed barley, wheat and sorghum to be tested, soak them in 50ml of methanol and stir for 2 minutes, let stand, and take the solution to obtain the samples to be tested . The resulting soaking solution is the sample to be tested. Others are with embodiment 1.

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Abstract

The invention discloses pesticide carbaryl artificial antigen and antibody and its producing method and application, the invention has carbamate pesticide, which has 6-amino caproic acid structure, micromolecule compound artificial hapten, antigen and antibody producing and its application in immunity analysis field. The invention opercomes the problems, such as fussy, high cost, low analyzing speed, of traditional physics and chemistry analyzing method, it provides convenient, rapid, sensitive, exact immunity analysis system. It uses 1-(5-carboxyl)-3-(1-naphthyl) carbamide and N-(2-naphthoyl)-6-amino caproic acid as hapten, it is connected with carrier albumen and horseradish peroxide enzyme to become artificial antigen and enzyme antigen. The artificial antigen is made to antibody after animal immunity, taking blood, separating antiserum, puring. The antibody is stable, it has good specificity and sensitivity, and the synthesizing method is simple. The invention can be used to pesticide carbaryl rapid detection, and it has good application foreground.

Description

technical field [0001] The invention belongs to the technical field of immunochemistry and residue analysis of small-molecule pesticide compounds (molecular weight less than 1000 Daltons); relates to organic synthesis, immunochemistry, biochemistry and physicochemical testing techniques, etc. Design and synthesis of artificial haptens and specific antigens of carbamate pesticide small molecule compound carbamate, and preparation of specific antibodies for immunized animals and their application in the establishment of immunoassay methods. Background technique [0002] Carbaryl (also known as carbaryl) is a representative product of carbamate pesticides. In my country, carbaryl can be used for 141 kinds of crops and control 565 kinds of pests. It is currently the most widely registered insecticide. Carbaryl is widely used in vegetables, cotton, and field crops. Carbaryl is used as a flower thinning agent on fruit trees, and it is used to control harmful organisms in fish pon...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/531A61K39/395A61K39/385G01N33/532G01N33/535G01N33/547
Inventor 王硕王俊平张燕
Owner TIANJIN UNIV OF SCI & TECH
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