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Antrodia mycelium active substance preparation method and its compositions

A technology of active substance and mycelium, which is applied in the field of preparation method and composition of the active substance of Shanzhi mycelium, can solve the problems of high price and slow growth speed, reduce cost and price, and remove DPPH free base, the effect of inhibiting the growth of cancer cells

Active Publication Date: 2008-05-21
GRAPE KING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Cedar tree and Cinnamomum camphora are native and rare tree species in Taiwan. The number of Antrodia camphorata and Cinnamomum chinensis that can grow on dead Cinnamomum camphora and Cedarwood is rare, and their growth rate is slow. The Chinese fir has been listed as a protected tree species by our government. It cannot be cultivated artificially and can only be obtained by wild collection. The price is extremely expensive. The fresh fruiting body of Antrodia camphorata is the cheapest at about NT$2,500-3,000 per tael wet weight. Shanzhi is cheaper, and it is an expensive traditional medicinal material in the Taiwanese market
[0006] At present, fruit bodies such as Shanzhi and Antrodia cannot be obtained by artificial cultivation, but Antrodia can be cultivated in solid or liquid state to produce Antrodia camphorata mycelium; mushrooms have no seeds in the liquid culture process with ventilation and stirring. Entities are produced, but mycelium exists in the form of spherical objects (peilets); therefore, it is a feasible and yet to be developed method to cultivate the mycelium of Cyanobacteria chinensis in a suitable liquid medium to produce a large amount of active substances of Chinese cypress. method

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  • Antrodia mycelium active substance preparation method and its compositions
  • Antrodia mycelium active substance preparation method and its compositions
  • Antrodia mycelium active substance preparation method and its compositions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1 Carry out the cultivation of mycelia with the mycelia of Shanzhi Zhi (CGMCC NO.1342, CGMCC NO.1343)

[0050] Mycelium strains:

[0051] They are strains CGMCC NO.1342 and CGMCC NO.1343 deposited in the General Microbiology Center of China Microbiological Culture Collection Management Committee.

[0052] Plate culture:

[0053] The mycelium was inoculated on a plate, and cultured at 25° C. for 13-15 days using a potato dextrin medium (Potato Dextrose Agar, PDA).

[0054] Flask culture:

[0055] Scrape the hyphae on the plate and inoculate in the flask, use the following medium, at about 25°C, pH 4.5, shake and culture on a shaker at a shaking speed of 10-250rpm for 7-9 days.

[0056] Medium formula:

[0057] Element Concentration (g / 100ml)

[0058] Grains (such as wheat flour) or

[0059] 2

[0060] Beans (such as soybean flour, mung bean flour, soybean flour, etc.)

[0061] Peptone (peptone) 0.1

...

Embodiment 2

[0071] Example 2 Separation of Active Components of Mycelium of Cyprinus chinensis--Use of Water Extraction

[0072] Isolation of mycelium and clarified liquid:

[0073] Adopt centrifugation method to separate the mycelia and clarified culture fluid of the Cedarwood mycelia gained in Example 1, use a conventional centrifuge, obtain from the Decater NX418S of Sweden ALFA LAVAL company; promptly separate the mycelia and clear liquid.

[0074] Isolation of the active substance from the mycelium:

[0075] Soak the above-separated mycelia in water, extract at 100°C for 1 hour, and then centrifuge to obtain the mycelium extract. The extraction can be repeated several times. Combine the mycelium extracts and add 3 times the volume of 95 % ethanol, precipitate overnight at 4°C, and finally separate the precipitate from the supernatant, take the precipitate as the active substance, freeze-dry the extracted active substance, and then redissolve it with double-distilled sterile wat...

Embodiment 3

[0080] Example 3 Separation of Active Components of Mycelium of Cyprinus chinensis--Using Organic Solvent Extraction

[0081] Using methanol to extract the active substances of Cedarwood mycelium:

[0082] Carry out freeze-drying of the liquid culture suspension of C. chinensis mycelia obtained in Example 1, including the mycelium and clarified liquid, to obtain the fermentation lyophilized powder of C. cedars. The lyophilized powder was shaken and extracted with 20 times the volume of methanol in a 1L shaker flask (120rpm, 16 hours, 15-30°C), and then the supernatant was obtained by centrifugation, and the residue was extracted once more in the same way as above, and the two The supernatant of the sub-extraction was concentrated to dryness by concentration under reduced pressure, redissolved in methanol and quantified to an appropriate concentration to obtain the methanol extract of Cedarwood mycelia, which was stored at 4°C for future use.

[0083] Using ethanol to extr...

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PUM

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Abstract

The invention discloses a process for preparing Antrodia salmonea mycelium active compound and the composition, wherein comprises inoculating Antrodia salmonea mycelium onto a plate, culturing 2 weeks at 25 deg C, inoculating bacterial filament in a flask, carrying out oscillation culture 7-9 days, inoculating flask culture into an acid fermenting tank, letting in air for culture, obtaining mycelium extract through solvent extraction, isolating mycelium active compound through ethanol deposition, carrying out vibration extraction to the culture suspending liquid with organic solvents such as ethanol and methanol, finally decompression concentrating the supernatant fluid.

Description

technical field [0001] The present invention relates to a method for preparing active substances of Antrodia salmonea mycelium and its composition, in particular to an Antrodia salmonea mycelium liquid cultured and extracted with a solvent. Preparation method and composition of silk active substance. Background technique [0002] Antrodia salmonea (Antrodia salmonea) is a fungus unique to this province, and it only parasitizes in the dry trunk of Cunninghamia konishii unique to Taiwan. Xiangshanzhi was officially named Antrodiasalmonea in 2004 (Zhang Dongzhu, 2004). It is a new species of Polyporaceae and the genus Antrodia. Basidiomycota, Basidiomycotina, Homobasidiomycetes, Aphyllophorales, Polyporaceae, Antrodia. [0003] Cunninghamia konishii, also known as Luan Dashan, is an evergreen coniferous tree unique to Taiwan Island. It can reach a height of 70 meters and a diameter at breast height of 2.5 meters. It is mainly distributed in the northern and central parts of T...

Claims

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Application Information

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IPC IPC(8): C12N1/14A61K36/07A61P35/00
Inventor 陈劲初许胜杰林宜瑾蔡美琳
Owner GRAPE KING
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