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Methods for extracting keratin proteins

a technology of keratin protein and extraction method, which is applied in the direction of peptide/protein ingredients, peptide sources, peptides, etc., to achieve the effect of minimizing the degradation of said keratin protein

Active Publication Date: 2015-03-19
KERANETICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The invention provides methods for extracting keratin proteins from keratin protein sources. This involves solubilizing the keratin proteins by treating them with an oxidizing or reducing agent, and then separating them from the keratin source by high-speed centrifugation to produce a clarified soluble keratin protein solution. The solution is then lyophilized to produce a keratin protein cake, which minimizes degradation of the keratin protein.

Problems solved by technology

The methods that have been described in the art to extract these proteins rely on a chemical process of oxidation or reduction with less than optimal extraction.

Method used

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  • Methods for extracting keratin proteins
  • Methods for extracting keratin proteins
  • Methods for extracting keratin proteins

Examples

Experimental program
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example 1

6.1 Example 1

Keratose Extraction Methods (Oxidative Extraction)

[0058]Untreated Chinese hair was used in the extraction method. The hair was end-cut to lengths of ¼, ½, ¾ and 1 inch segments and cleaned by washing in a warm water solution.

[0059]Step 1: The hair was added to a mixing tank. The tank was a 316L stainless steel vessel that contained a propeller for mechanical agitation (see FIG. 1). The oxidant was added to the vessel. The oxidant used was a 2% solution of paracetic acid (PAA) at a 25:1 weight-to-weight ratio. The mixture was mechanically mixed for a period of 12 hours at 37° C. The mechanical mixing resulted in complete oxidation of the hair shafts (see FIG. 2).

[0060]Step 2: The residual solution containing the oxidant was drained, neutralized and discarded.

[0061]Step 3: The oxidized hair was collected and rinsed with water until PAA test strips revealed no residual oxidant in the solution.

[0062]Step 4: A base was then added to the drained hair in a ratio of 25:1. In th...

example 2

6.2 Example 2

Kerateine Extraction Methods (Reductive Extraction)

[0073]Kerateine Extraction Methods (Reductive Extraction).

[0074]Untreated Chinese hair was end-cut to lengths of ¼, ½, ¾ and 1 inch segments and washed in a warm water solution.

[0075]Step 1: The hair was added to a 316L stainless steel vessel that contained a propeller for mechanical agitation (see FIG. 1). The reductant was added to the vessel. The reductant was a 0.5M solution of a thioglycolic acid (TGA) at a ratio of 25:1. The mixture was mechanically mixed for a period of 15 hours at 37° C. The mechanical mixing can be beneficial to improve the extent to which reduction occurs in the hair shafts.

[0076]Step 2: The solution containing reductant and extracted keratin proteins was collected and stored in a separate container at 4° C. The remaining hair was retained by sieving through a steel mesh with a pore size of 400 microns. The mechanical agitation applied during the straining process helps to collect as much solu...

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Abstract

Described herein are methods to produce keratin protein-based biomaterials, the parameters required to achieve improved extraction, the parameters required to improve isolation, the parameters of lyophilization and the grinding process to achieve consistent particulate sizes of protein materials.

Description

1. FIELD OF THE INVENTION[0001]This invention relates to methods to extract and purify keratin protein-based biomaterials.2. BACKGROUND OF THE INVENTION[0002]Keratins are a family of proteins found in the hair, skin, and other tissues of vertebrates. Hair is a unique source of human keratins because it is one of the few human tissues that are readily available and inexpensive. Although other sources of keratins are acceptable feedstocks for the present invention (e.g. wool, fur, horns, hooves, beaks, feathers, scales, and the like), human hair is preferred because of its biocompatibility in human medical applications.[0003]Keratins can be extracted from human hair fibers by oxidation or reduction using methods that have been widely published in the art. If one employs a reductive treatment, the resulting keratins are referred to as kerateines. If an oxidative treatment is used, the resulting keratins are referred to as keratoses. These methods typically employ a two-step process whe...

Claims

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Application Information

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IPC IPC(8): C07K1/36C07K14/47
CPCC07K14/4741C07K1/36
Inventor BURNETT, LUKEBOYD, SARAH ANN
Owner KERANETICS
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