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Cell culture device having culture medium replacement function

Active Publication Date: 2015-01-08
JTEC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention is a cell culture apparatus with a culture medium replacement function that can automatically shift top lids from petri dishes and discharge old culture medium using a suction tube, inject new culture medium using an ejection tube, cover petri dishes with top lids, and continue cell culture. The apparatus is downsized and simplified in structure, reducing the cost and burden on researchers and operators. Multiple stages of petri dishes and top lids can be set simultaneously, and the apparatus provides high space efficiency. The apparatus also includes a synchronous rotation means and a rotary shaft body for easy opening and closing of petri dishes and top lids, as well as for advanced cleaning and sterilization treatment of the lower disc and upper disc. The apparatus also includes an opening / closing door and cutout portions for easy setting of petri dishes and top lids.

Problems solved by technology

Therefore, researchers and operators have been forced to bear heavy labor and burden.
However, because a multijoint industrial robot is extremely expensive, an automatic cell culture apparatus is also a large-scale and expensive system and therefore cannot be easily utilized.

Method used

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  • Cell culture device having culture medium replacement function
  • Cell culture device having culture medium replacement function
  • Cell culture device having culture medium replacement function

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first embodiment

[0055]Next, the present invention will be described in more detail on the basis of embodiments illustrated in the accompanying drawings. FIGS. 1 and 2 illustrate a cell culture apparatus having a culture medium replacement function according to the present invention. FIGS. 3 to 6 illustrate discs which constitute a holder for holding Petri dishes and top lids. In the drawings, reference numeral 1 denotes an incubator chamber, 2 denotes a drive chamber, 3 denotes a partition wall, 4 denotes a Petri dish, 5 denotes a top lid, 6 denotes an upper Petri dish, 7 denotes a top lid, 8 denotes a rotary shaft body, 9 denotes a bearing unit, 10 denotes a holder, 11 denotes a drive motor, 12 denotes a linear actuator, and 13 denotes culture medium replacement means.

[0056]In the present invention, as illustrated in FIGS. 1 and 2, the incubator chamber 1 and the drive chamber 2 located above the incubator chamber 1 are arranged with the partition wall 3 interposed therebetween. The rotary shaft b...

second embodiment

[0071]Next, a cell culture apparatus having a culture medium replacement function according to the present invention will be described with reference to FIGS. 14 to 28. In the drawings, reference numeral 101 denotes an incubator chamber, 102 denotes a drive chamber, 103 denotes a partition wall, 104 denotes a Petri dish, 105 denotes a top lid, 106 denotes a lower disc, 107 denotes an upper disc, 108 denotes a rotary shaft body, 109 denotes a bearing unit, 110 denotes a holder, 111 denotes a drive motor, 112 denotes a linear actuator, and 113 denotes culture medium replacement means.

[0072]Also in the present embodiment, a basic operation is the same as the operation in the first embodiment. Specifically, the cell culture apparatus of the present embodiment has the following configuration. At least a set of an upper disc 107 and a lower disc 106 each fixed to a rotatable shaft is horizontally arranged inside the incubator chamber 101. The Petri dishes 4 in each of which cells and a cu...

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Abstract

Provided is a small-scale cell culture apparatus having a culture medium replacement function for culturing cells in a small number of Petri dishes, the cell culture apparatus being capable of performing automatic replacement of the culture medium and cell culture within the same cavity and significantly reducing the apparatus cost. At least one set of upper and lower discs is arranged inside an incubator chamber 1. Petri dishes 4, 6 in each of which cells and a culture medium are put are held on lower discs 18, 19, and top lids 5, 7 are held on upper discs 19, 30. The lower side of each of the top lids is open.

Description

TECHNICAL FIELD[0001]The present invention relates to a cell culture device having a culture medium replacement function, and more specifically to a cell culture device having a culture medium replacement function for culturing cells or fungi in a Petri dish.BACKGROUND ART[0002]Conventionally, cells have been cultured by repeatedly performing the following operation. A culture medium and cells are put in a Petri dish, the Petri dish is housed inside an incubator in which environment such as temperature and atmospheric gas is controlled with a top lid closed to thereby culture the cells, the Petri dish is taken out of the incubator at every predetermined period and transferred to an operation space in which dispensation and separation are performed, the culture medium is replaced in the operation space, and the Petri dish is again returned to the incubator. When a culture medium replacement operation is manually performed, it is necessary not only to pay careful attention to prevent ...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12M1/26
CPCC12M33/04C12M25/06C12M23/10C12M29/00C12M41/14C12M41/48C12M99/00C12M23/50C12M29/10
Inventor TSUMURA, TAKASHIONO, TAKAHIRO
Owner JTEC
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