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Manipulating plant sensitivity to light

a plant sensitivity and light technology, applied in the field of plant genomics and plant improvement, can solve the problems of not providing one with a means to determine, insufficient homolog identification to make specific predictions, etc., and achieve the effects of improving yield and growth, increasing or decreasing activities, and improving vigor

Inactive Publication Date: 2014-12-25
MONSANTO TECH LLC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent identifies genes that affect how plants respond to light. Manipulating these genes can improve crop yield and make plants more tolerant to stress. The genes can also affect the levels of certain molecules and the timing of leaf senescence. The disclosed genes can be used to create transgenic plants with better light sensitivity, higher yield, and better ability to cope with abiotic stresses. The patent also provides methods for selecting and producing these transgenic plants.

Problems solved by technology

However, in many cases, the identification of homologs is not sufficient to make specific predictions because not all homologs have the same function.
Thus, an initial analysis of functional relatedness based on sequence similarity alone may not provide one with a means to determine where similarity ends and functional relatedness begins.

Method used

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  • Manipulating plant sensitivity to light
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[0154]It is to be understood that the present disclosure is not limited to the particular devices, machines, materials and methods described. Although particular embodiments are described, equivalent embodiments may be used to produce the instant compositions and practice the instant methods.

[0155]The disclosure, now being generally described, will be more readily understood by reference to the following examples, which are included merely for purposes of illustration of certain aspects and embodiments of the present disclosure and are not intended to limit claims to the instant compositions or methods. It will be recognized by one of skill in the art that a polypeptide that is associated with a particular first trait may also be associated with at least one other, unrelated and inherent second trait which was not predicted by the first trait.

example i

Vector and Cloning Information for Arabidopsis

[0156]A number of constructs were used to modulate the activity of the disclosed sequences. An individual project was defined as the analysis of lines for a particular construct (for example, this might include any of the disclosed lines that constitutively overexpress a disclosed B-box polypeptide sequence). In the present study, a full-length wild-type version of a gene was directly fused to a promoter that drove its expression in transgenic plants. Such a promoter could be the native promoter of that gene, or the CaMV 35S promoter. Alternatively, a promoter that drives tissue specific or conditional expression could be used in similar studies.

[0157]In the present study, expression of a given polynucleotide from a particular promoter was achieved by either a direct-promoter fusion construct in which that sequence was cloned directly behind the promoter of interest, or by a two-component expression system. A direct fusion approach has ...

example ii

Transformation

[0159]Gruber et al., 1993, described several expression vectors and culture methods that may be used for cell or tissue transformation and subsequent regeneration.

Dicot and Monocot Expression Vectors

[0160]Transformation of plants and plant parts by Agrobacterium-mediated transformation can be practiced using a recombinant DNA construct, vector or cassette with the genetic elements as shown in Table 5 for dicots and Table 6 for monocots. The disclosed recombinant polynucleotides in the Sequence Listing “MBI—0120 PCT_ST25” may be incorporated in such recombinant DNA constructs. The elements in a recombinant DNA construct provided in Tables 5 and 6 are only examples of different elements that can be used for the purpose of producing transgenic plants that have incorporated the polynucleotides. Other elements can be used as well in combination with the polynucleotides of the present disclosure to achieve trait modification.

[0161]A base recombinant DNA construct specificall...

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Abstract

The present disclosure identifies new genes which have the potential to increase broad acre yield in crops. This disclosure is based upon our fundamental knowledge of light signal transduction and our understanding of the roles these genes play in regulating plant growth and development in response to light. Transgenic plants with gain- or loss-of-function of one of these genes, or in combination, are expected to show significant improvements in broad acre yield and stress tolerance.

Description

CROSS REFERENCE TO RELATED APPLICATION[0001]This application claims priority to U.S. Provisional Application Ser. No. 61 / 488,592 (filed on May 20, 2011), which is incorporated by reference herein in its entirety.JOINT RESEARCH AGREEMENT[0002]The claimed invention, in the field of functional genomics and characterization of plant genes for the improvement of plants, was made by or on behalf of Mendel Biotechnology, Inc. and Monsanto Company as a result of activities undertaken within the scope of a joint research agreement in effect on or before the date the claimed invention was made.RELEVANT FIELD[0003]The present disclosure relates to plant genomics and plant improvement.BACKGROUND[0004]A plant's traits, including its biochemical, developmental, or phenotypic characteristics that enhance yield or tolerance to various abiotic stresses, may be controlled through a number of cellular processes. One important way to manipulate that control is through transcriptional regulators—protein...

Claims

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Application Information

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IPC IPC(8): C12N15/82
CPCC12N15/8261C12N15/8241C12N15/8273C12N15/8243C12N15/8229C12N15/8245C12N15/8223C12N15/8271C12N15/8225C12N15/8246C07K14/415Y02A40/146
Inventor KHANNA, RAJNISHKRONMILLER, BRENT A.RATCLIFFE, OLIVER J.REUBER, T. LYNNEPETRACEK, MARIE E.MEISTER, ROBERT J.PREUSS, SASHAMCCLERREN, AMANDAQI, QUNGANG
Owner MONSANTO TECH LLC
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