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Method of preparing an artificial tooth primordium in vitro and artificialtooth primordium derived therefrom

Inactive Publication Date: 2014-12-25
TECHNICAL UNIVERSITY OF BERLIN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides an artificial tooth primordium and a method of producing it without the need for any artificial biological or non-biological scaffold or carrier. The method involves culturing isolated mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate that exhibits the markers and functions of a tooth primordium. The isolated dental pulp cells can be subjected to non-adherent culture for at least one passage prior to being subjected to non-adherent conditions. The method is efficient in inducing differentiation of dental tissue and can provide an artificial tooth primordium that is suitable for transplantation. The invention allows for the production of artificial tooth primordia that are derived from the cells of the person to be treated with the artificial tooth primordium, reducing the risk of rejection reactions. The method can also be used to screen substances that can influence tooth or tooth tissue development.

Problems solved by technology

However, even under optimal dental care impairment, destruction or loss of teeth can occur.
However, these tooth replacement therapies require a significant surgical procedure and, eventually, laborious follow-up care.
In addition, these synthetic implants do not meet all demands on a tooth like e.g. an “absorption” by the natural periodontal ligament which fixes the tooth within the jawbone.
However, in most of these approaches embryonic cells are used, the cells are cultured under non-physiological adherent culture conditions and require the presence of a synthetic scaffold or carrier.

Method used

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  • Method of preparing an artificial tooth primordium in vitro and artificialtooth primordium derived therefrom
  • Method of preparing an artificial tooth primordium in vitro and artificialtooth primordium derived therefrom
  • Method of preparing an artificial tooth primordium in vitro and artificialtooth primordium derived therefrom

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Embodiment Construction

[0055]General Methods

[0056]Mesenchymal Dental Pulp Cell Isolation

[0057]Dental pulp cell isolation is performed according to a modified protocol from Gronthos et al. (A method to isolate and culture expand human dental pulp stem cells. Methods Mol Biol. 2011; 698:107-21).[0058]1 Extracted third molars from patients after informed consent are collected in DMEM containing 10% FCS and penicillin and streptomycin (100 μg / ml each) and stored at 4° C. for not longer than 24 hours.[0059]2 The biopsies are handled under sterile conditions throughout the whole procedure of cell extraction. Tooth crown and the root if present are wiped with 100% ethanol. To open the pulpal cavity, the tooth is split by mechanical cracking with a hammer.[0060]3 Pulp tissue is removed with forceps and placed into a PBS containing petri dish.[0061]4 The tissue is then cut into small fragment which are then washed twice with PBS to remove debris and blood.[0062]5 Afterwards a digestion step is performed with a col...

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Abstract

The present invention is directed to a method of preparing an artificial tooth primordium in vitro, comprising the steps: a) providing isolated mesenchymal dental pulp cells; and b) culturing the mesenchymal dental pulp cells under non-adherent conditions to form a cell aggregate representing an artificial tooth primordium; as well as to an artificial tooth primordium derived therefrom.

Description

BACKGROUND OF THE INVENTION[0001]Dental care is the best way of maintaining healthy denture. However, even under optimal dental care impairment, destruction or loss of teeth can occur. Such impairment, destruction or loss of teeth may be e.g. disease-related, hormone-related, therapy-related or a consequence of an accident. Nowadays, in such a case dental implants are used which usually are formed of materials comprising metal (e.g. titan), ceramics and / or composites. These implant techniques are highly sophisticated and from a medical and cosmetic point of view achieve already high levels. However, these tooth replacement therapies require a significant surgical procedure and, eventually, laborious follow-up care. In addition, these synthetic implants do not meet all demands on a tooth like e.g. an “absorption” by the natural periodontal ligament which fixes the tooth within the jawbone.[0002]Thus, it is an aim in the field of regenerative medicine to provide means which allow the ...

Claims

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Application Information

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IPC IPC(8): C12N5/077A61L27/38G01N33/68A61K35/32G01N33/50
CPCC12N5/0654A61K35/32G01N33/5044G01N33/6887A61L2430/12A61L27/3821C12N2506/1361C12N2502/094C12N2513/00A61L27/3865C12N5/0664A61L27/3804A61L27/38G01N33/50
Inventor LAUSTER, ROLANDMARX, UWEBINDER, JENNIFERROSOWSKI, MARK
Owner TECHNICAL UNIVERSITY OF BERLIN
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