Nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof and a detection method using the same

Inactive Publication Date: 2013-11-07
SEOUL NAT UNIV R&DB FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The present invention provides nanoparticles that can be used to assemble various aligned and unconventional nanostructures. These nanoparticles have a head part and a body part and are highly useful for detecting DNA and analyzing the onset and progression of particular diseases.

Problems solved by technology

However, synthesizing and assembling these complex nanostructures are challenging, and the use of multicomponent, multimeric metallic nanostructures is severely limited because of their synthetic inaccessibility.

Method used

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  • Nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof and a detection method using the same
  • Nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof and a detection method using the same
  • Nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof and a detection method using the same

Examples

Experimental program
Comparison scheme
Effect test

example 1

Materials and Methods

[0084]Materials

[0085]All the chemical reagents [AgNO3, polyvinylpyrrolidone (MW 40,000 and 10,000 K value: 29-32), (+)-sodium L-ascorbate, hydroxyl amine, dithiothreitol and sodium dodecyl sulfate] were purchased from the Sigma-Aldrich (St. Louis, Mo., USA) and used without further purification. Au nanoparticles (AuNPs) were purchased from Ted Pella, Inc. (Redding, Calif., USA). HPLC-purified oligonucleotides and NAP-5 column were purchased from IDT, Inc. (Coralville, Iowa, USA) and GE Healthcare (Sephadex G-25 medium, DNA grade), respectively. NANOpure water (>18.0 MQ, Milli-Q) was used for all the experiments.

[0086]Methods

[0087]HR-TEM analysis

[0088]The formvar / carbon-coated copper grid (Ted Pella, Inc. Redding, Calif., USA) and HR-TEM (JEOL, Japan, 300 kV) were used for the HR-TEM analysis.

[0089]Preparation of DNA-modified gold nanoparticles Oligonucleotides on gold nanoparticles (AuNPs) were modified and characterized based on literature procedures (Hurst, S....

example 2

Preparation of Au-Ag head-body nanoparticles with the shape of nanosnowman

[0091]The nanosnowman structures were synthesized by using DNA-AuNPs prepared in Example 1 as seeds and adding Ag precursors and other reagents to grow Ag structures on the DNA-AuNP surface.

[0092]Au-Ag head-body nanosnowman structures were synthesized using 1% polyvinylpyrrolidone (PVP), 0.1 M (+)-sodium L ascorbate (L-SA) and 1 mM silver nitrate (AgNO3) in deionized water by a polymer-aided chemical reduction method. The PVP, L-SA, and Ag precursors were added sequentially to the DNA-modified AuNP seed solution prepared in Example 1. The molar ratio between the reducing agent and Ag+ (L-SA / Ag) was kept at 50 and the molar ratio between the number of PVP repeating units and Ag+ (PVP / Ag) was kept at 30. Typically, 100 μL of 0.2 nM DNA AuNPs was reacted with 59.4 μL of 1 mM AgNO3 solution in the presence of 19.8 μL of 1% PVP and 29.7 μL of 0.1 M L-SA at room temperature, respectively. The resulting mixture was m...

example 3

Structural changes of nanoparticles depending on salt concentration

[0093]When the salt concentration was varied from high to very low values (0.3 M, 0.1 M, and 0.003 M NaCl), different solution colors ranging from yellow to orange, dark-green, and bright green were observed (FIG. 1a), along with a structural transformation from spherical Au-Ag core-shell particles to Au-Ag head-body nanosnowman particles.

[0094]The formed nanostructures were confirmed by high-resolution transmission electron microscopy (HR-TEM; JEOL, Japan, 300 kV) and UV-vis spectroscopy (Agilent 8453 spectrophotometer, USA). FIG. 1b shows the HRTEM images of nanostructures obtained from reactions with different salt concentrations. FIG. 1b shows the structural changes from spherical particles to snowman-like nanostructures. The UV-vis data (FIG. 1b-4) show that the plasmonic peaks of the nanostructures were strongly affected by varying the salt concentration, with a new plasmonic band appearing at 630 nm at a very ...

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Abstract

The present invention relates to nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof, and a detection method using the same. More particularly, the present invention relates to nanoparticles in the shape of nanosnowman with head and body parts, which can offer platforms for DNA-based assembly of various aligned and unconventional nanostructures and is highly applicable to the detection of DNA and an analyte associated with the onset and progression of a particular disease, a preparation method thereof, and a detection method using the same.

Description

BACKGROUND OF THE INVENTION[0001]1. Field of the Invention[0002]The present invention relates to nanoparticles in the shape of nanosnowman with a head part and a body part, a preparation method thereof, and a detection method using the same. More particularly, the present invention relates to nanoparticles in the shape of nanosnowman with head and body parts, which can offer platforms for DNA-based assembly of various aligned and unconventional nanostructures and is highly applicable to the detection of DNA and an analyte associated with the onset and progression of a particular disease, a preparation method thereof, and a detection method using the same.[0003]2. Description of the Related Art[0004]Because of their plasmonic, catalytic, electronic, and magnetic properties, metal nanostructures have been intensively studied over the past decade. Combining multiple metallic nanocomponents into a single specific nanostructure often generates unusual optical and chemical properties such...

Claims

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Application Information

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IPC IPC(8): G01N21/65
CPCG01N21/658B82B1/00B82B3/00G01N21/62G01N33/54346G01N33/553G01N2021/653
Inventor NAM, JWA MINLEE, JUNG HOONOH, JEONG WOOK
Owner SEOUL NAT UNIV R&DB FOUND
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