Beverage Packed in Sealed Container
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example 1
(1) Theanine Preparation 1
[0031] Theanine was prepared based on the technique disclosed as an embodiment in gazette of Japanese Patent No. 2139750. More specifically, 0.3 M glutamine and 1.5 M ethylamine were reacted in the presence of 0. 3U / ml glutaminase (refined from pseudomonas nitroreducens IFO 12694) at 30° C. for 22 hours in a boric acid buffer fluid (Na2B4O7—NaOH, pH 11) , whereby theanine was obtained. Reaction liquid was applied to Dowex 50×8 column chromatgraphy and Dowex 1×2 column chromatography thereby to be eluted by ethanol, whereby theanine was refined. As a result, 5 kg L-theanine was obtained. In the following description, the theanine prepared by the above-described preparing method will be referred to as “theanine 1.”
(2) Theanine Preparation 2
[0032] 1500 kg water was warmed up to 80° C., and 100 kg green tea leaves were added to the warmed water and stirrered for 30 minutes so that tea dregs were removed. Thereafter, filtration and concentrated refinement we...
example 2
(1) Preparation of Tea Beverage:
[0035] Extraction from 1 kg tea leaves of green tea was carried out in 10 liter hot water (60° C.) for 30 minutes and thereafter, solid-liquid separation was carried out by a filtration process, whereby 7 liter green tea extract was obtained.
[0036] Theanine 1, Theanine 2, quinic acid and tea extract powder containing quinic acid were blended together with blending quantities of the respective substances shown by TABLE 1 and put into a polyethylene terephthalate (PET) bottle aseptically after ultra high temperature (UHT) sterilization (sterilization at 135° C. for 3 seconds), whereby a beverage contained in a sealed container was prepared. Thus, 8 types of test beverages (1 to 8) and two types of comparative beverages (9 and 10; comparative example) were prepared.
TABLE 1ComparativeTest beveragebeverage12345678910Theanine 1 (mg)100100300300100300Theanine 2 (mg)5001000Quinic acid (mg)50100501005050Tea extract100010001000powder (mg)Green tea450450450...
example 3
Black Tea Beverage Contained in a Sealed Container
(1) Preparation of Black Tea Beverage:
[0052] Extraction from 1 kg tea leaves of black tea was carried out in 10 liter hot water (60° C.) for 30 minutes and thereafter, solid-liquid separation was carried out by a filtration process, whereby 7 liter black tea extract was obtained.
[0053] Theanine 1, Theanine 2, quinic acid and black tea extract powder containing quinic acid were blended together with blending quantities of the respective substances shown by TABLE 4 and put into a PET bottle aseptically after ultra high temperature (UHT) sterilization (sterilization at 135° C. for 3 seconds), whereby a beverage contained in a sealed container was prepared. Thus, 6 types of test beverages (11 to 16) and two types of comparative beverages (17 and 18; comparative example) were prepared.
[0054] Furthermore, contents of theanine and quinic acid in each beverage were analyzed in the same manner as in the embodiment 2.
TABLE 4ComparativeT...
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