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Metabolomic determination in assisted reproductive technology

a technology of assisted reproductive technology and metabolic analysis, which is applied in the field of metabolic determination in assisted reproductive technology, can solve the problems of inability to the visual appearance of these cells does not correlate well with viability, and the existing tools for their discovery, identification and quantification do not meet the needs of researchers or clinicians, etc., and achieves low-cost instruments, easy to use, and rapid analysis

Inactive Publication Date: 2007-07-12
MCGILL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The patent text describes a technology platform called biospectroscopy-based metabolomics (BSM) that combines two scientific disciplines to analyze biomarkers and molecular diagnostics. This technology has broad application in human and animal health, including reproductive health, and can be used to non-invasively monitor the health and disease status of cells, tissues, and organs. It can also be used to identify viable cells, sperm, and embryos for in vitro fertilization procedures. The BSM platform is simple, accurate, and cost-effective, and can provide valuable decision-making capability in multiple clinical settings. The patent text also describes a method for determining the metabolomic profile of a sample, which can be used to predict the success of in vitro fertilization procedures and related methods.

Problems solved by technology

With the completion of the sequencing of the human genome, it has become apparent that genetic information alone is incapable of providing a comprehensive characterization of the biochemical and cellular functioning of complex biological systems.
Because proteins and metabolites are far more numerous, diverse, and fragile than genes, existing tools for their discovery, identification, and quantification do not meet the needs of researchers or clinicians.
Visual appearance of these cells does not correlate well with viability in many cases.
No other information about what is going on in the cells is available to ART specialists to guide the process.
While genetic testing of embryos by extracting one cell is possible to determine at least some factors of viability, such an “invasive” procedure can adversely affect the embryo.
No technique presently exists that allows embryologists and other ART specialists to efficiently assess the viability of these cells for ART procedures.
Today there are no practical biological criteria or analytical methods enabling selection of oocyte, sperm, or embryo to ensure efficacy or safety of the IVF procedure.
Consequently, with no analytical methodologies available to reliably assess oocyte competency at the front end of the IVF procedure, embryologists have resorted to subjective and non-standardized embryo development criteria and morphology as indicators of embryo quality and, by inference, original oocyte quality.

Method used

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  • Metabolomic determination in assisted reproductive technology
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Examples

Experimental program
Comparison scheme
Effect test

example 1

Measurement of OS from Short Wavelength Near Infrared (SWNIR) Spectroscopy of Blood Plasma

Sampling Methodology

[0110]Prior to analysis samples were thawed for one hour to reach room temperature and then centrifuged for 30 min. For cleaning and preconditioning, the sample cell was first rinsed with 200 μl of 0.1 M NaOH followed by 3×200 μl Millipore water.

Short Wavelength Near Infrared (SWMIR) Spectroscopy

[0111]A SWNIR spectrum was recorded of the third water rinse serving as a control. Thereupon, 75 μl of sample was injected into the sample cell and a sample spectrum recorded using the apparatus as shown in FIG. 1. Short wavelength near infrared spectra were obtained from the prepared samples using the following protocol. For the measurements, an American Holographic near infrared spectrophotometer was used. The spectrophotometer is equipped with a two channel input port so that a reference could be obtained simultaneous with the measurement sample. Spectra acquired covered the 580 t...

example 2

[0130]The exemplary methods of Example 1 may be employed in IVF procedures, and in monitoring fetal-maternal health. The methods are also applicable to harvesting oocytes, sperm, and embryos, as well as the cryopreservation of same. In vitro fertilization programs employing these methods are also contemplated.

example 3

[0131]Metabolomic signatures of the oxidative stress constituents in the developing embryo are also obtained using analytical separation techniques which identify nutrient and metabolite fractions of the media. An example of a separation using capillary electrophoresis with ultraviolet optical detection is given in FIG. 7. Constituents associated with embryo development are identified and have both positive and negative correlations with embryo viability. In particular, one nutrient fraction at 900 seconds is negatively correlated with embryo viability while two metabolite fractions (1000-1100 sec) are positively correlated. Combining the information from the both constituent groups, separation of embryos which result in pregnancy and those which did not, is achieved with a sensitivity of 80% and a specificity of 100%. Using the same methodology developed here, separations techniques such as, other electromotive separation techniques, liquid and gas chromatography, can also be used ...

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Abstract

The present invention relates to a method for determining the ideal time for and outcome of reproductive health procedures including in vitro fertilization by establishing a correlation between the successful outcome of said procedure and the spectra of a body fluid obtained using a chosen analytical modality for a population of patients, acquiring for a patient a spectrum of the body fluid of the patient using said chosen modality.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims priority on provisional application Ser. No. 60 / 757,242, filed Jan. 9, 2006, the disclosure of which is herein incorporated by reference.TECHNICAL FIELD[0002]The present invention relates to determining a state of a cell exchanging metabolites with a fluid medium by analyzing the metabolites in the fluid medium, and to uses thereof.BACKGROUND OF THE INVENTIONMetabolomics[0003]With the completion of the sequencing of the human genome, it has become apparent that genetic information alone is incapable of providing a comprehensive characterization of the biochemical and cellular functioning of complex biological systems. As a result, the focus of much molecular biological research is shifting toward proteomics and metabolomics, broadly defined as the systematic analysis of proteins and small molecules such as polypeptides, hormones and neurotransmitters (and their metabolites) in a physiological environment such as a ...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/08C12N5/06G16B20/40
CPCG01N21/31G01N21/359G01N21/65G01N33/5091G01N33/689G06F19/18G01N2021/651G01N2201/1293G01N2800/367G01N2800/368G01R33/465G01N2021/6482G16B20/00A61P15/00G16B20/40
Inventor BURNS, DAVID HUGH
Owner MCGILL UNIV
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