Microbial feedstock for filter feeding aquatic organisms
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example 1
[0020] To test the nutritional value of bacteria, and the procedure of culturing, the following experiment was done. Two 200-liter glass aquaria were set up out doors. The aquaria were partially shaded. The aquaria were filled with seawater from the Red Sea. On the first day, each aquarium received 10 mg of Artemia cysts from the Great Salt Lake, and iron EDTA, urea, and sodium dibasic phosphate. The final concentrations within the aquaria were as follows: ferric chloride 0.002 gms per liter, sodium EDTA 0.0035 gms per liter, sodium dibasic phosphate 0.0025 gms per liter, and urea 0.05 gms per liter. On day three, ten grams of sucrose were added to aquarium 1, and none to aquarium 2. On day 23, the Artemia were harvested with a fine aquarium net, daubed dry, and weighted. From tank 1 (with sugar) 27.0 grams of Artemia were harvested, and from tank 2 (without sugar) 3.0 grams of Artemia were harvested. The temperature during the time of the experiment ranged from approximately 25 to ...
example 2
[0024] The next illustrative example was done indoors to control the temperature. With air conditioning the room temperature was maintained at approximately a constant 27 degrees centigrade. On day 1, each of three plastic buckets were set up with fifty liters each of sea water from the Red Sea. Each bucket received 60 mgms of Artemia cysts from the Great Salt Lake, and nitrogen, phosphorus, and iron as in the previous experiment. The buckets were aerated as in the previous experiment. On the second day, buckets 2, and 3 received 2.5 Gms of sucrose, and 50 ml of an overnight culture of bacteria. The bacterial culture was prepared by taking 500 ml of fresh sea water, and adding iron, nitrogen, and phosphorus as before and 0.2 gms of sugar per liter, and aerating overnight. Overnight the cultures became quite cloudy. On the 5th day, bucket 3 received 2.5 Gms of sugar, and nitrogen, phosphorus and iron equivalent to what had been added initially. Bucket 3 also received 50 ml of bacteri...
example 3
[0028] A further illustrative example was undertaken with four plastic buckets with 50 liters each. Along with the sea water as noted above, nitrogen, phosphorus, iron and about 60 mgs of Artemia cysts were set up as in the previous illustrative examples. On the second day, buckets 2, 3 and 4 were given 2.5 Gms of sugar (10 ml of 0.25 Gms per cc in water). Also added to the buckets was 50 ml of overnight bacterial culture. On the 3rd day, bucket 2 was illuminated with a 100 watt light bulb suspended about six inches above water surface. The light was kept on for the duration of the experiment. On the 5th day, 2.5 gms of sucrose was added to buckets 2, 3 and 4. On the 5th day, fifty ml of bacteria culture was added to all buckets. On the 9th day, 2.5 Gms of sucrose were added to buckets 2, 3 and 4 and fifty ml of bacteria culture was also added to all buckets. On the 12th day, 2.5 gms of sugar was added to buckets 2, 3 and 4 and all buckets received 50 ml of bacterial culture and nit...
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