Composition having bacteristatic and bactericidal activity against bacterial spores and vegetative cells and process for treating foods therewith
a technology of which is applied in the field of compositions having bacteristatic and bactericidal activity against bacterial spores and vegetative cells and process for treating foods therewith, can solve the problems of spoilage, illness, death, etc., and achieve excellent bacteristatic and bactericidal activity
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example 1
Inhibition of Antibacterial Composition Against Bacillus cereus in Whole and Skim Milk at 30° C.
[0063] Whole and skim milk was sterilized, 2,3,5-Tripheryltetrazolium chloride (TTC) was added (for indicating growth by color change), and the resulting mixture was inoculated with spores of Bacillus cereus (an aerobic sporeformer) at about 4-5 log cells / ml. The antibacterial composition (CB-1) and a Novasin™ control (Control) were added as 10% stock solutions (the Control contained 4 times the amount of nisin as CB-1). The inhibition was indicated by the minimal concentration for no growth during a given time and is set forth below in Table 2.
TABLE 2Effectiveness of CompositionsMIC* (% of antibacterialcomposition required forNovasin ™ concentrationTreatmentno growth)(ppm)In whole milkControl>4>3200CB-13600In skim milkControl0.5400CB-12400
*MIC = minimum inhibition concentration
[0064] The plate count of B. cereus for these compositions was then obtained on TSA agar plates, incubated a...
example 2
Inhibition of Antibacterial Composition Against L. monocytogenes in Whole and Skim Milk at 25° C.
[0065] Whole and skim milk was sterilized, 2,3,5-Tripheryltetrazolium chloride (TTC) was added (for indicating growth by color change), and the resulting mixture was inoculated with L. monocytogenes ATCC 19115 (a vegetative, gram positive pathogen) at about 4-5 log cells / ml. The antibacterial composition (CB-1) and Novasin™ control (Control) were added as 10% stock solutions (the Control contained 4 times the amount of nisin as CB-1). The inhibition was indicated by the minimal concentration for no growth during a given time and is set forth below in Table 4.
TABLE 4Effectiveness of CompositionsMIC* (% ofantibacterialcompositionrequired for noTreatmentgrowth)Novasin ™ concentration (ppm)In whole milkControl43200CB-12400In skim milkControl0.5400CB-11100
*MIC = minimum inhibition concentration
[0066] The plate count of L. monocytogeties for these compositions was then obtained on TSA agar...
example 3
Inhibition of Antibacterial Composition Against C. sporogenes, in Whole Milk at 30° C.
[0067] Whole milk was sterilized, 2,3,5-Tripheryltetrazolium chloride (TTC) was added (for indicating growth by color change), and the resulting mixture was inoculated with C. sporogenes (an anaerobic, nonpathogenic sporeformer) at about 4-5 log cells / ml. The antibacterial composition (CB-1) and a Novasin™ control (Control) were added as 10% stock solutions (the Control contained 4 times the amount of nisin as CB-1). The plate count of C. sporogenes for these compositions was then obtained on TSA agar plates, incubated at 30° C. for 24 hours and the results are indicated in Table 6 below.
TABLE 6TreatmentCFU / mlControl2 × 10e8Novasin6 × 10e6CB-12 × 10e4
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