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Compositions and methods for controlling stem cell and tumor cell differentiation, growth, and formation

a stem cell and tumor cell technology, applied in the field of self-assembling peptide amphiphiles, can solve the problems of cell proliferation and tumorigenicity of embryonic stem cells, and achieve the effects of reducing reducing size, and preventing additional tumor formation or metastasis

Inactive Publication Date: 2005-09-29
NORTHWESTERN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0006] The present invention also provides methods comprising the steps of: a) providing a peptide-amphiphile composition; and a subject, wherein the subject has a tumor; and b) contacting the peptide-amphiphile composition with the subject under conditions such that the subject's tumor is decreased in size or such that additional tumor formation or metastasis is reduced or prevented.

Problems solved by technology

However, embryonic stem cells can be tumorigenic.

Method used

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  • Compositions and methods for controlling stem cell and tumor cell differentiation, growth, and formation

Examples

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example 1

Protection of ESC-Transplanted Mice Against Tumor Formation.

[0035] This Example describes the protection of embryonic stem cell (ESC) transplanted mice against tumor formation in the presence of peptide amphiphiles (IKVAV-peptide amphiphile).

A. Methods

Animal Model of Focal Brain Ischemia

[0036] Under anesthesia (Avertin, 0.2 ml / 10 gram body weight, i.p.), an incision was made between left ear and eye in adult female mice (129 sv, 4-8 w). A craniotomy was performed, and the middle cerebral artery was exposed. Thereafter, the left middle cerebral artery was cauterized, and the incision was sutured by a 3-0 silk surgery suture.

Embryonic Stem Cell Preparation

[0037] Embryonic stem cells (R1, a cell line derived from 129sv mice) were propagated in DMEM defined medium (20% fetal bovine serum, 0.1 mM of non-essential amino acids, 50 μg / ml of penicillin-streptomycin, 0.1 mM of L-glutamine, 1000 U / ml of leukemia inhibitory factor, 100 μM of β-mercaptoethanol). The cells were harveste...

example 2

Transplantation of Glioma Cells (9L) with Self-Assembling Gel Under the Skin

[0042] This example describes the use of peptide-amphiphile gels to inhibit tumor formation in vivo.

Preparation of Glioma Cells In Vitro

[0043] Gliosarcoma (9L) cells, derived from Fischer 344 rats, are grown in DMEM medium with 10% fetal bovine serum, and are collected with 0.05% trypsin-EDTA. The cells are washed with HBSS twice and 107 cells are re-suspended with 300 μl IKVAV-PA or glucose (vehicle control) or HBSS.

Transplantation of Glioma Cells with Self-Assembling Gel

[0044] Tumor cells are suspended with IKVAI-PA or glucose. The cells are then injected subcutaneously in the shaved left flanks of anesthetized rat (female Fischer 344, 100˜150 g. Measurement of tumor formation is performed weekly.

Transplantation of Glioma Cells Followed by Self-Assembling Treatment

[0045] Glioma cells are re-suspended with HBSS at a concentration of 107 cells / 300 μl, and are then injected into the shaved right fl...

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Abstract

The present invention relates to the use of self-assembling peptide amphiphiles to prevent tumor formation by transplanted stem cells. The present invention further relates to the use of self-assembling peptide amphiphiles to treat cancers.

Description

[0001] This application claims priority to provisional patent application Ser. No. 60 / 532,249, filed Dec. 23, 2003, which is herein incorporated by reference in its entirety.FIELD OF THE INVENTION [0002] The present invention relates to the use of self-assembling peptide amphiphiles to prevent tumor formation by transplanted stem cells. The present invention further relates to the use of self-assembling peptide amphiphiles to treat cancers. BACKGROUND OF THE INVENTION [0003] Embryonic stem cells are pluripotent and can give rise to virtually all cell lineages in the body. Thus there is a great clinical potential for using embryonic stem cells to replace damaged cells in any given organ or tissue. However, embryonic stem cells can be tumorigenic. Therefore, a major concern for using embryonic stem cells is tumor formation after grafting. What is needed are compositions and methods useful in preventing tumor formation after embryonic stem cell grafting. SUMMARY OF THE INVENTION [0004]...

Claims

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Application Information

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IPC IPC(8): A61K35/48A61K38/08A61K45/00A61K45/06
CPCA61K45/06A61K35/48A61P35/00
Inventor ZHAO, LI-RUKESSLER, JOHN A.
Owner NORTHWESTERN UNIV
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