Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Resolution method for axis chiral enantiomers of lesinurad

a chiral enantiomer and axial enantiomer technology, applied in the field of pharmaceuticals, can solve the problems of low total yield, low optical purity, and inability to industrialize the resolution method by using instruments, and achieve the effects of low cost, low cost, and simple filtration

Active Publication Date: 2022-06-21
CHINA RESOURCES SAIKE PHARMA
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0038]The method of the invention uses optically pure quinoline natural products and derivatives thereof as chemical resolving agents to chemically resolve lesinurad racemate. The axial chiral enantiomer of lesinurad in R configuration can be obtained with a chiral purity ee of up to 100% and a total yield of 90% or more. The axial chiral enantiomer of lesinurad in S configuration can be obtained with a chiral purity ee of up to 99.9% and a total yield of 80% or more. Furthermore, the chiral resolving agents used are cheap and easy to obtain, can be conveniently recovered and reused, thus reducing the separation cost and facilitating industrial production.
[0039]For example, when quinine or cinchonine is used for resolution, the method of the invention has multiple advantages compared with the prior art, and the details are shown below:

Problems solved by technology

For the current level of industrial production, the resolution method by using instruments is costly and cannot be industrialized.
However, the isomers obtained by the method have low optical purities and low total yields, the chiral resolving agents are expensive and the economic cost is high.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Resolution method for axis chiral enantiomers of lesinurad
  • Resolution method for axis chiral enantiomers of lesinurad
  • Resolution method for axis chiral enantiomers of lesinurad

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0079]Racemic lesinurad (2-(5-bromo-4-(4-cyclopropylnaphthalen-1-yl)-4H-1,2,4-triazol-3-ylthio)acetic acid) was synthesized according to the method described in patent documents WO2009070740A2, CN103524440A or WO2014008295A1. The specific synthetic route is as follows:

[0080]

4-(1-cyclopropylnaphthalen-4-yl)-4H-1,2,4-triazol-thiol (compound A, 14.5 g, 54.2 mmol) was dissolved in 150 ml DMF, potassium carbonate (11.2 g, 81.3 mmol) was added to the solution, and then methyl bromoacetate (5.88 g, 54.2 mmol) was added dropwise. After the addition was complete, the solution was raised to 50° C. and kept for 4 hours. After the reaction was complete, the reaction solution was partitioned between water (100 ml) and ethyl acetate (100 ml). The aqueous phase was extracted with ethyl acetate (2×100 ml). The organic phases were combined, washed with saline (6×150 ml), dried over anhydrous sodium sulfate for 1 h, and filtrated with suction. The filtrate was evaporated to dryness to obtain 15.6 g w...

example 2

[0083]

[0084]Salt formation: 10 g of lesinurad racemate (1.0 eq.) was added to ethyl acetate (100 ml). The mixture was heated to 70° C., and quinine (8.1 g, 1.0 eq.) was slowly added under stirring. The mixture was heated to reflux, and kept at the constant temperature for 1 h. The reaction system was slowly and naturally cooled down to room temperature, then kept in an ice-water bath and stirred for 30 mins. After filtration, the filter cake was washed with ethyl acetate (20 ml) to obtain 9.1 g white solid powder A (salt of lesinurad in R configuration), ee=97.9%. The filtrate was concentrated by rotary evaporation and dried to obtain 9.0 g white solid powder C (salt of lesinurad in S configuration), ee=94.5%.

[0085]Recrystallization: 9.1 g of the above white solid powder A was added to ethyl acetate (90 ml) under stirring. The mixture was heated to reflux, and kept at the constant temperature for 1 h. The reaction system was slowly and naturally cooled down to room temperature, then...

example 3

[0088]

[0089]Salt formation: 10 g of lesinurad racemate (1.0 eq.) was added to toluene (100 ml). The mixture was heated to 105° C., and cinchonine (7.3 g, 1.0 eq.) was slowly added under stirring. The mixture was heated to reflux, and kept at the constant temperature for 1 h. The reaction system was slowly and naturally cooled down to room temperature, then kept in an ice-water bath and stirred for 30 mins.

[0090]After filtration, the filter cake was washed with toluene (20 ml) to obtain 8.9 g white-like solid (salt of lesinurad in S configuration, a crude product), ee=80%. The filtrate was concentrated by rotary evaporation and dried to obtain 9.1 g white solid powder (salt of lesinurad in R configuration, a crude product), ee=40%.

[0091]Recrystallization: 8.9 g of the above white-like solid (salt of lesinurad in S configuration, a crude product) was added to toluene (90 ml) under stirring. The mixture was heated to reflux, and then kept at the constant temperature for 1 h. The reacti...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
temperatureaaaaaaaaaa
chiral purity eeaaaaaaaaaa
chiral purity eeaaaaaaaaaa
Login to View More

Abstract

A resolution method of axial chiral enantiomers of lesinurad (2-(5-bromo-4-(4-cyclopropylnaphthalen-1-yl)-4H-1,2,4-triazol-3-ylthio)acetic acid) adopts inexpensive and readily available quinoline natural products and derivatives thereof, such as quinine, cinchonine, quinidine or cinconidine as resolving agents to react with lesinurad racemate in an organic solvent to form a salt, and the salt is dissociated by acidification so as to obtain optically pure (R)- or (S)-2-(5-bromo-4-(4-cyclopropylnaphthalen-1-yl)-4H-1,2,4-triazol-3-ylthio)acetic acid. The method can give axial chiral enantiomer of lesinurad in R configuration with a chiral purity ee of up to 100% and a total yield of 90% or more. The obtained axial chiral enantiomer of lesinurad in S configuration can reach a chiral purity ee of up to 99.9% and a total yield of 80% or more.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]The application is a U.S. national entry of PCT International Application No. PCT / CN2019 / 084640, filed Apr. 26, 2019, which claims the priority to the earlier application No. CN201810444393.3 submitted to China National Intellectual Property Administration on May 10, 2018, which is entitled “Resolution method of axial chiral enantiomers of lesinurad”. The earlier application is incorporated herein by reference in its entirety.FIELD OF THE INVENTION[0002]The invention belongs to the pharmaceutical field, and relates to a resolution method of axial chiral enantiomers of lesinurad (i.e. 2-(5-bromo-4-(4-cyclopropylnaphthalen-1-yl)-4H-1,2,4-triazol-3-ylthio)acetic acid).BACKGROUND OF THE INVENTION[0003]Lesinurad, i.e. 2-(5-bromo-4-(4-cyclopropylnaphthalen-1-yl)-4H-1,2,4-triazol-3-ylthio)acetic acid, CAS:878672-00-5, having a structure as shown in Formula 2, is an oral effective inhibitor of uric acid transporter 1 (URAT1) for the treatment of ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(United States)
IPC IPC(8): C07D249/12
CPCC07D249/12C07B57/00C07B2200/07A61K31/4196A61P19/06
Inventor BIAN, DEQIANLIU, MOYIYANG, YANCHI, FANGZE
Owner CHINA RESOURCES SAIKE PHARMA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com