Microorganism feed addictive improving reproduction ability of boar and making method thereof
A microbial feed and additive technology, applied in the field of feed additives, can solve problems such as the decline of semen quality and quantity, the insignificant improvement of economic benefits, and the impact on the reproductive performance of sows, so as to enhance the anti-stress ability, improve the anti-stress ability, The effect of preventing free radical damage
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Embodiment 1
[0037] The preparation method of the microbial feed additive for improving the reproductive performance of boars adopts strains obtained through screening and optimization, and adopts a process of combining pure breed fermentation and compound fermentation at the same time, which includes the following steps:
[0038] Step 1: Transfer the strains into the broth medium respectively, and after culturing at 35°C for 24 hours, select the colonies with obvious colony characteristics, strong vigor and fast growth and reproduction under the microscope to separate them by streaking be preserved;
[0039] The second step: Lactobacillus lactis is inoculated in the liquid medium, and the liquid medium is composed of: soybean meal 3%, glucose 1%, yeast extract 1%, magnesium sulfate heptahydrate 0.05%, potassium dihydrogen phosphate 0.03%, water 94.92%, the pH is adjusted to 5.0, the culture temperature is 30°C, and the time is 20h, to obtain the culture of Lactobacillus lactis;
[0040] ...
Embodiment 2
[0045] Step 1: Transfer the strains into the broth medium respectively, and after culturing at 35°C for 24 hours, select the colonies with obvious colony characteristics, strong vigor and fast growth and reproduction under the microscope to separate them by streaking be preserved;
[0046] The second step: Lactobacillus lactis is inoculated in the liquid medium, and the liquid medium is composed of: soybean meal 4%, corn steep liquor 2%, glucose 1.2%, yeast extract 1.2%, magnesium sulfate heptahydrate 0.03%, dihydrogen phosphate Potassium 0.06%, water 91.5%, pH adjusted to 5.0, culture temperature 35°C, time 24 hours, to obtain Lactobacillus lactis culture;
[0047] The third step: Bacillus subtilis is inoculated in the solid medium, and the solid medium is composed of: bran 74%, rice husk 12%, corn flour 6%, soybean meal 7.94%, magnesium sulfate 0.05%, manganese sulfate 0.01%, The pH is adjusted to 6.4, the culture temperature is 39° C., and the time is 38 hours to obtain a ...
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