Sample separation, identification, dispensing method and its device and analysis device

A technology for separating devices and distances, applied in sampling devices, analysis materials, measuring devices, etc., can solve the problems of high sample mortality rate and unreliable guarantee of normal state

Active Publication Date: 2011-12-07
雅马拓科学株式会社
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, for example, when living cells are used as a sample example, there is a problem that the death rate of the sample after dispensing is high, and even a living sample cannot be reliably guaranteed to be in a normal state.

Method used

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  • Sample separation, identification, dispensing method and its device and analysis device
  • Sample separation, identification, dispensing method and its device and analysis device
  • Sample separation, identification, dispensing method and its device and analysis device

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Embodiment Construction

[0206] Hereinafter, embodiments of the present invention will be described with reference to the accompanying drawings. In addition, the sample referred to in the present invention refers to particulate objects, such as organic substances such as cells, etc., with a size of about 0.1 μm to 500 μm, inorganic substances such as polystyrene, foamable substances, magnetic substances, etc., metals and other substances. , or, to indicate their state of suspension in liquid. In addition, a sample indicates a substance that has the presence or absence or degree of identification elements marked, or a substance that emits light naturally. In this case, the sample may be identified by the presence or absence of a plurality of identification elements or a combination of degrees, and a plurality of samples having a plurality of identification elements may exist. In addition, the so-called agglutinated sample refers to a substance having a size of about 0.2 μm to 10 mm in which the sample...

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PUM

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Abstract

In the separation part, the separation state can be confirmed, in the detection part, the irradiation efficiency and light receiving sensitivity are improved, and in the dispensing part, the sample is guaranteed to be in a normal state. A sample separation device, characterized by comprising: a container containing a sample; a nozzle for sucking and discharging a sample from the container; a nozzle operating mechanism for moving the nozzle up, down, left, and right; A nozzle control mechanism that controls the suction force and discharge force of the nozzle. In addition, a sample identification device is characterized by comprising: a nozzle for sucking a sample from a container containing the sample; a flow path for flowing the sample; and monitoring light irradiation for observing (monitoring) the sample. The unit and the light receiving unit are provided in the measurement unit of the flow channel, and the nozzle, the flow channel and the measurement unit are integrally formed.

Description

technical field [0001] The present invention relates to a separation method, identification method, and dispensing method of a sample (agglutinated sample), and an apparatus and analysis apparatus for implementing each method. Background technique [0002] A conventional cell sorter used for the separation, identification, and dispensing of samples is roughly composed of a separation unit, a detection unit, and a dispensing unit. [0003] First, refer to Figure 41 The separation part will be described. First, in order to homogenize the aggregated sample 243 in the test tube 241, the test tube 241 is vibrated, or the inside of the test tube 241 is stirred. Next, suction and discharge 251 of the agglutinated sample 243 in the test tube 241 are repeated using the pipette 245 . The aggregated sample 247 is subjected to shear stress due to repeated suction and discharge 251 , and thus is separated into a single sample 249 . In this method, the single sample located on the sur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/10G01N21/53G01N21/64G01N35/02G01N35/10B07C5/00
CPCG01N15/1459G01N15/1436B01F11/0074G01N2035/1062G01N1/286B07C5/00B01F5/0057G01N15/1427G01N2015/1075B01F25/10B01F31/651
Inventor 高桥亨月井健徐杰
Owner 雅马拓科学株式会社
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