Method for pre processing sample in use for measuring concentration of salvianolic acid B, in biologic body fluid

Abstract

A method for pre-treating analysis sample of red phenolic acid B concentration in body fluid on living beings includes utilizing water- methanol to spray-wash then utilizing aqueous ammonia-methauol to elute Waters Oasis HLB small column obtained by activating sample containing red phenolic acid with water and methanil, enriching eluted liquid, using high efficiency of chromatography to carry out pretreatment on living beings sample containing red phenolic acid B after enriched matter is redissloved by flowing phase of 1% trifluoroacetic acid: methanol: acetonitrile.

Description

Technical field [0001] The invention relates to the pharmacokinetics research field of traditional Chinese medicines, in particular to the pharmacokinetics research field of salvianolic acid B and Chinese medicines containing salvianolic acid B, in particular to a pretreatment method for measuring the concentration of salvianolic acid B in biological fluids. Background technique [0002] Salvia miltiorrhiza is one of the most commonly used Chinese medicinal materials. The research results show that the water-soluble components are mainly responsible for promoting blood circulation and removing blood stasis, while salvianolic acid B has the highest content of water-soluble components and the strongest activity of activating blood circulation and removing blood stasis. The National Pharmacopoeia Commission designated salvianolic acid B as the quality control indicator for Danshen tablets in March 2002. Furthermore, salvianolic acid B may become a quality control indicator for Dansh...

AI Technical Summary

Problems solved by technology

Therefore, there is insufficient understanding of the pharmacokinetics of salvianolic acid B and salvianolic acid B in Danshen preparations, which cannot provide a basis for the development of new salvianolic acid B drugs and guide clinical rational drug use.

[0005] In addition, since salvianolic acid B is a water-soluble component, when the concentration in the sample is low, it is difficult to fully extract salvianolic acid B in the sample by the traditional liquid-liquid extraction method

Therefore, the method that prior art adopts, the sensitivity of detection is low, can't detect the blood drug concentration when low concentration in pharmacokinetic research

[0006] Therefore, due to the particularity of the chemical properties of salvianolic acid B, if the pretreatment method adopted is not suitable, the sensitivity of the concentration determination method will be insufficient.