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Method for promoting proliferation of hematopoietic cell

A technology of hematopoietic cells and hematopoietic stem cells, applied in X-ray/γ-ray/particle irradiation therapy, artificial respiration, physical therapy, etc., can solve the problems that no one has proved the influence of donor cells

Inactive Publication Date: 2007-05-09
THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Prior to the present invention, although many studies have shown that low-dose radiation has a proliferative effect on the hematopoietic system, so far, no one has demonstrated the effect of these donor cells on the recipient bone marrow hematopoietic system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1: Determining the radiation dose that produces the optimal stimulating effect

[0021] Preparation of bone marrow cell suspension; BALB / C male mice with an average body weight of 18-22 g were killed by dislocation and soaked in 75% alcohol for 3-5 minutes. Then, the bilateral femurs were cut under aseptic conditions, and the phenol red-free RPMI-1640 culture solution was sucked out with a syringe with a 7-gauge needle, and then the bone marrow cavity was flushed and single cells were routinely isolated. After counting the number of bone marrow mononuclear cells with a peripheral blood cell counter (Beckmen Co.), the cell density was adjusted to 1×10 6 / mL.

[0022] Donor bone marrow cells were irradiated with 6MV X-rays generated by a linear accelerator (Philips Co.). The source-to-skin distance (SSD) used for irradiation was 100cm, the irradiation field area was 10cm×10cm, and the low-dose irradiation dose rate was 3cGy / min.

[0023] Take 21 culture flasks,...

Embodiment 2

[0033] Example 2: Effect of irradiated donor bone marrow cells on recipient hematopoietic reconstitution

[0034] Preparation of bone marrow cell suspension: BALB / C male mice with an average body weight of 18-22 g (provided by the Animal Experiment Center of Soochow University) were used as donor animals, and they began to drink gentamicin (320 mg / L) + erythromycin (250mg / L) water for injection until 2 weeks after transplantation. Recipient mice are female clean grade BALB / C mice.

[0035] Animals were sacrificed by dislocation, soaked in 75% alcohol for 3-5 minutes, aseptically scissored both femurs, sucked phenol red-free RPMI-1640 culture solution with a syringe with a 7-gauge needle, rinsed the bone marrow cavity and isolated single cells, and then used A blood cell counter (Beckmen Co.) measured the number of bone marrow mononuclear cells in each femur by counting white blood cells.

[0036] The 6MV X-rays produced by a linear accelerator (Philips Co.) were used for lo...

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Abstract

A method for promoting the reproduction of hematopoietic cell includes such steps as irradiating the bone marrow cells or hematopoietic stem cells of donor by low-dosage ray, and conventionally transplating the irradiated bone marrow cells into receptor.

Description

technical field [0001] The invention relates to a method for rebuilding bone marrow hematopoietic function, in particular to a method for promoting the proliferation of bone marrow hematopoietic cells in a recipient by utilizing the stimulation effect of low-dose radiation. The method includes performing external low-dose radiation irradiation on donor bone marrow cells or hematopoietic stem cells in advance, and then infusing the donor bone marrow cells irradiated outside the body into the recipient. Background technique [0002] Since the 1980s, research on the excitatory effects of low-dose radiation has become one of the hotspots in the field of radiation medicine research. People have conducted extensive and in-depth research on low-dose radiation to stimulate cell proliferation and enhance the immune function of the body (Luckey TD. Nurture with ionizing radiation: a provocative hypothesis. Nutrition and cancer, 1999, 34: 1-11; Lin et al., Effects of low-dose radiatio...

Claims

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Application Information

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IPC IPC(8): A61N5/10A61N1/36
Inventor 张力元江家贵
Owner THE SECOND HOSPITAL AFFILIATED TO SUZHOU UNIV
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