Giardia sporangiocyst in water and Cryptosporidium hominis egg capsule checking method

Abstract

The present invention relates to a giardia cyst in water and a cryptosporidium oocyst checking method which belonging to the detection technique field, the giardia and the cryptosporidium in water are detected by the combined-dyeing of haematein and intensified kinyoun after the filtration by microporous membrane and the gradient separation by acetic ether and formalin in a ratio of 10:1. The background complexity of the following microscopic examination is alleviated by membrane filtering the water sample and the giardia and the cryptosporidium are separately dyed by the combined-dyeing of haematein and intensified kinyoun. The invention alleviates the impurity interference in the background in traditional giardia and cryptosporidium detecting method and improves the microscopic examing confirmation accuracy of the morphological and structural features.

Description

Technical field [0001] The invention relates to a detection method in the technical field, in particular to a detection method for Giardia cysts and Cryptosporidium oocysts in water. Background technique [0002] Giardia and Cryptosporidium are a type of intestinal protozoa that parasitize humans and animals. Among the causes of human water-borne intestinal-borne diseases in developed countries, protozoa are one of the main causes of water-borne transmission. The main protozoa that pollute drinking water are Giardia intestines and Cryptosporidium. At present, the most authoritative US EPA1623 detection method for the two insects is expensive and requires high experimental conditions. For example, the 1623 method involves a large number of expensive consumables, such as special filter capsules, immunomagnetic bead separation kits, fluorescent staining kits, DAPI, etc. These are all disposable consumer products and cannot be recycled. Taking into account the current specific nation...

AI Technical Summary

Problems solved by technology

Among the results: for membrane-IMS-GiemSa staining, since the filter capsule was changed to filter membrane, and the fluorescent staining was changed to Giemsa staining, the cost was about 60% lower than that of capsule-IMS-IFA, and the experimental conditions were also lower. It does not require expensive equipment such as fluorescence microscopes, but the results must be observed by experienced experimenters to reduce errors, and the cost is still relatively high; while membrane-gradient separation-Giemsa staining is a traditional detection method, which is easy to operate, The cost is very low, only equivalent to 10% of the capsule-IMS-IFA, but due to the lack of specific separation by IMS, the background of the stained film is more complex, there are more algae and impurities interference, and the microscopic examination of the morphological and structural features has certain limitations difficulty