Novel extraction method of bromelain

A technology of bromelain and a new method, applied in directions such as hydrolase, can solve the problems of rough product, need to be improved in purity, large energy consumption, etc., and achieve the effects of improving purity and yield, shortening drying time, and improving use efficiency

Inactive Publication Date: 2007-02-07
谢丹青 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, chemical methods are mainly used for extraction in my country. The chemical methods are outdated, the products are rough, the content of impurities is high, and the enzyme activity is not high.
Patent CN96114240.5 provides a physical extraction method of bromelain. The raw material of pineapple plant is crushed and squeezed after being frozen and pretreated (the temperature is -5°C--20°C, and the time is 20-30 hours) (the pH of the juice should be adjusted to 4.0-4.7), after the juice is filtered and separated, use membrane technology for ultrafiltration and reverse osmosis to obtain purified and concentrated enzyme liquid, and the finished product of the enzyme liquid is directly freeze-dried and vacuum-dried (ie pineapple plant raw material → freezing pretreatment → crushing and squeezing → filtration and separation →ultrafiltration→reverse osmosis→freeze vacuum drying→finished product), the enzyme activity of bromelain produced by this method is relatively low, and the purity needs to be improved. It is also a process step that consumes a lot of energy. The entire production process is relatively cumbersome and the production cost is high, which is not conducive to large-scale industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] The new method for physical extraction of bromelain in this embodiment comprises the following steps: raw material→cleaning→coarse crushing→fine crushing→pretreatment→juicing→centrifugation→freezing→filtering→ultrafiltration 1→ultrafiltration 2→reverse osmosis→vacuum Dry → finished product. Specifically: use pineapple fruit, leaves, skin, and stems as raw materials, first wash off the sediment on the raw materials with clean water, crush the raw materials into 5mm particles with a coarse grinder, and then crush them into 0.1mm particles with a fine grinder . Add 1% sodium metabisulfite as an antioxidant and 1% sodium benzoate as a bacteriostatic agent to the crushed fine particles, then use a screw juicer to obtain juice, and then use a disc centrifuge to separate the juice, take the supernatant, and remove the residue discarded. Then add 0.2% EDTA, 1% cysteine ​​and a small amount of filter aid to the supernatant, stir thoroughly for 30 minutes to remove heavy metals...

Embodiment 2

[0025] The new method for physical extraction of bromelain in this embodiment comprises the following steps: raw material→cleaning→coarse crushing→fine crushing→pretreatment→juicing→centrifugation→freezing→filtering→ultrafiltration 1→ultrafiltration 2→vacuum drying→finished product . Specifically: use pineapple leaves, skins, and stems as raw materials, first wash away the sediment on the raw materials with clean water, use a coarse grinder to grind the raw materials into 10mm-sized particles, and then use a fine grinder to grind them into 3mm particles. Then add 2% sodium metabisulfite as an antioxidant and 5% sodium benzoate as a bacteriostatic agent in the fine particles, then squeeze the juice with a hydraulic juicer to obtain juice, and then separate the juice with a three-legged centrifuge to get the supernatant, Discard the slag. Then add 0.5% EDTA, 0.5% cysteine ​​and a small amount of filter aid to the supernatant, stir thoroughly for 60 minutes to remove heavy metal...

Embodiment 3

[0027] The new method for physical extraction of bromelain in this embodiment includes the following steps: raw material→cleaning→coarse crushing→fine crushing→pretreatment→juicing→centrifugation→freezing→filtration→ultrafiltration 1→reverse osmosis→vacuum drying→finished product. Specifically: use pineapple leaves, skins, and stems as raw materials, first wash away the silt on the raw materials with clean water, use a coarse grinder to grind the raw materials into 8mm-sized particles, and then use a fine grinder to grind them into 1mm particles. Then add 1.5% sodium metabisulfite as an antioxidant and 3% sodium benzoate as a bacteriostatic agent in the fine particles, and then squeeze the juice with a rotary knife juicer, then separate the juice with a horizontal continuous centrifuge, and take the supernatant liquid, discard the slag. Then add 0.5% EDTA, 0.8% cysteine ​​and a small amount of filter aid to the supernatant, stir for 40 minutes to remove heavy metals and impuri...

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Abstract

the invention discloses a new physical extracting method of pineapple proteinase, which comprises the following steps: cleaning raw material; grinding roughly; grinding finely; predisposing; squeezing; centrifuging; freezing; filtering through two-grade; proceeding two-grade hyperfiltration; hyperfiltrating; drying in the vacuum to obtain the product.

Description

technical field [0001] The invention relates to a method for extracting bromelain, in particular to a new method for extracting bromelain from pineapple plants using a physical method. Background technique [0002] Bromelain is a kind of proteolytic enzyme, a pure natural plant protease produced by biotechnology from the fruit, fruit stalk, peel, fruit leaf, stem leaf and stem of the pineapple plant. Bromelain is light gray to light yellow powder rich in pineapple aroma. Bromelain has a strong effect on decomposing fibrin and blood clots, and can effectively improve human immunity, so it has a significant medical effect. Bromelain can carry out various biochemical reactions such as protein hydrolysis, and bromelain can be widely used in food, biology, medicine, cosmetics and other industries. At present in my country, chemical methods are mainly used for extraction. The chemical methods are backward in technology, rough in product, high in impurity content, and low in enzy...

Claims

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Application Information

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IPC IPC(8): C12N9/50
Inventor 刘锋
Owner 谢丹青
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