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Pretreatment method for lily tissue culture bulbs before transplanting them

A lily group and pretreatment technology, applied in the field of plant tissue culture, can solve the problems of slow germination, weak growth, irregular seedling emergence, etc., and achieve the effects of vigorous seedling growth, strong stress resistance, and improved stress resistance.

Inactive Publication Date: 2007-01-31
潘利军
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current lily tissue culture seedlings are directly transferred after simply reducing the humidity and hardening before transplanting. After the tissue culture seedlings are transferred, the germination is slow, the emergence is irregular, and the growth is weak

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 100

[0011] The method of embodiment 1 lily tissue culture seedling low temperature treatment:

[0012] 1) Place lily bulblets with a diameter of 0.8-1.5 cm (average 1.3 cm) induced during tissue culture under low temperature conditions of 0, 2, 4, 6, 8, and 10°C before being transferred to the ground. After 30 days of treatment, , transferred into the same substrate, and observed its growth situation, as shown in Table 1 below;

[0013] temperature(℃)

No pretreatment group CK

0

2

4

6

8

10

Number of seeds planted (pieces)

100

100

100

100

100

100

100

Emergence time after planting (days)

20

15

10

10

13

15

20

Seedling growth

weak

generally

it is good

better

better

generally

weak

Uniformity of emergence

not good

generally

it is good

it is good

better

generally

not...

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PUM

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Abstract

A method for pre-treating the tissue-cultured bulb of lily before transplanting features that the bulb of lily is treated at 2-6 deg.C for 30-60 days, resulting in high survival rate and strong seedling.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a pretreatment method before transferring lily tissue culture balls. Background technique [0002] Plant tissue culture refers to the method of inoculating a part of a plant, such as a small stem, a small leaf, or even a cell, on a medium under in vitro conditions to make them regenerate into a complete plant. The steps of tissue culture include disinfection of medium and inoculation material, inoculation, plant induction, rooting and transplanting. [0003] Present lily tissue-cultured seedlings are directly transferred after simply reducing the humidity to harden the seedlings before transplanting. After the tissue-cultured seedlings are transferred, germination is slow, seedlings are not neat, and their growth is weak. Contents of the invention [0004] The object of the present invention is to provide a pretreatment method for lily tissue culture balls with fast germination, neat se...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 潘利军
Owner 潘利军
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