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Macroporous support for chemical amplification reactions

A macroporous carrier and amplification reaction technology, applied in organic chemistry, laboratory containers, chemical instruments and methods, etc., can solve the problems of expensive manufacturing, complicated maintenance of precise distribution system, and inapplicability of precise distribution system, achieving Effect of avoiding temperature gradient and reducing cost

Inactive Publication Date: 2006-11-15
INFINEON TECH AG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Such precision dispensing systems are complex to maintain and expensive to manufacture
Especially for service and general laboratory surroundings, precise dispensing systems are not suitable

Method used

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  • Macroporous support for chemical amplification reactions
  • Macroporous support for chemical amplification reactions
  • Macroporous support for chemical amplification reactions

Examples

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Embodiment Construction

[0079] Figure 1 shows a cross-sectional view (A) of an embodiment of a device according to the invention made of, for example, glass, Al 2 o 3 or silicon, based on a macroporous support material in which (1) is the pore wall, (2) is the pore, and (3) the dried compound that forms a predetermined portion of the reaction mixture that can be used in an amplification reaction; ( B) shows a cross-sectional view of an embodiment of a device according to the invention based on locally oxidized macroporous silicon, wherein (3) is a pre-treatment for forming a reaction mixture that can be used for an amplification reaction The specified portion of the dried compound, (4) is the silicon core of the pore wall, (5) is SiO 2 And (6) is composed of SiO 2 The wall of the hole made; the cross-sectional view of the embodiment shown in figure (A) has a cover (7) arranged on the lower surface side, which is based, for example, on elastomers and also acts as a seal, while figure ( D) is then a...

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Abstract

A method for carrying out an amplification of nucleic acids in pores of a two-dimensionally designed macroporous support material according to one embodiment includes the step of providing a predetermined part of a reaction mixture necessary for the amplification of a nucleic acid in pores of the support material. A device for carrying out the amplification of nucleic acids according to one embodiment includes a two-dimensionally designed macroporous support material having a multiplicity of pores, wherein a predetermined part of a reaction mixture for carrying out an amplification of nucleic acids is provided in the pores.

Description

technical field [0001] The invention relates to a method for amplifying nucleic acids in the pores of a planar macroporous carrier material, wherein two or more pores of the carrier material are provided with a mixture of reaction mixtures required for nucleic acid amplification. Predetermined section. Furthermore, the invention also relates to a device comprising a planar, macroporous carrier material having a large number of pores, wherein a predetermined portion of the reaction mixture for carrying out the nucleic acid amplification is provided in the pores. Background technique [0002] Methods including the study of nucleic acids are increasingly used in molecular biology research, but also in the diagnosis of a large number of diseases. For this purpose, the corresponding nucleic acids are generally amplified. Polymerase chain reaction (PCR) is an in vitro method for the controlled amplification of fragments of DNA. Short oligonucleotides (primers) bind specifically...

Claims

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Application Information

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IPC IPC(8): C12P19/34C07H21/00
CPCB01L3/50857B01L2200/16B01L2300/069B01L2300/0819B01L2300/0893C12Q1/686C12Q2523/308C12Q1/6876
Inventor S·德尔廷格M·克吕尔T·哈内德
Owner INFINEON TECH AG
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