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Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence

A drug-resistant and specific combination technology for gastric cancer, applied in the field of biomedicine

Inactive Publication Date: 2006-11-08
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

However, the 200510042810.4 patent only simply screens for polypeptides that specifically bind to cells, and does not specifically screen for polypeptides that affect cell functions

Method used

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  • Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence
  • Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence
  • Screening method for human gastric cancer drug-resistant cell specific combination and drug-resistant reverse short peptide and sequence

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Embodiment Construction

[0145] Drug-resistant cell surface molecular markers in gastric cancer are one of the key targets for gastric cancer multidrug resistance reversal therapy. A group of human gastric cancer drug-resistant cells were successfully obtained through in vitro whole-cell screening of phage random peptide library combined with MTT in vitro drug sensitivity test function identification. The specific binding of cells and the reversal of drug-resistant short peptide GMBPs, and the use of phage cell binding experiments, immune cell / histochemical staining, cell binding competition inhibition experiments, immunofluorescence detection and other methods to identify the binding specificity of GMBPs, and further use peptide MTT In vitro drug sensitivity test and in vivo drug sensitivity test on nude mice confirmed the reverse drug resistance characteristics of GMBPs. The specific methods are as follows:

[0146] 1. Technical solution

[0147] 1.1. In vitro whole-cell subtractive screening of pha...

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Abstract

The present invention belongs to the field of biomedicine technology, and is screening method and sequence for human gastric cancer drug resistant cell specific combination and drug resistant reverse short peptide. The present invention features that through the combined random bacteriophage peptide library and MTT extracorporeal drug sensitive test screening, bacteriophage cloning polypeptide capable of reversing the drug resisting characteristic of the drug resistant gastric cancer cell is obtained to lower the survival rate and IC50 value of drug resistant gastric cancer cell under chemotherapy medicine and result in statistic difference cloning (p<0.05). Bacteriophage polypeptide is utilized in screening whole cell and the ligand of specific expression molecule on drug resisting tumor cell membrane, so as to the bioactive polypeptide and corresponding bioactive short peptide and its sequence (GMBPs).

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and specifically relates to a screening method and sequence of short peptides for specific binding of drug-resistant cells of human gastric cancer and drug-resistance reversal. Background technique [0002] Gastric cancer is the malignant tumor that causes the largest number of deaths in my country. Chemotherapy is one of the main methods for the treatment of gastric cancer, but there is still no significant progress in its therapeutic effect. The main reason is that gastric cancer cells develop multidrug resistance to chemotherapy drugs. Multi-drug resistance (MDR) of tumor cells refers to the cross-resistance of tumor cells to other chemotherapeutic drugs with different chemical structures and mechanisms after developing resistance to a certain chemotherapeutic drug. [0003] Current studies have shown that the generation of multidrug resistance in tumor cells is mainly related to the follo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68G01N33/574G01N33/68
Inventor 林涛丁杰梁树辉韩全利吴开春樊代明
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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