A kind of tumor chemotherapeutic drug preparation combination
A chemotherapeutic drug and a chemotherapeutic drug technology, which is applied in the field of combination of tumor chemotherapeutic drug preparations, can solve the problems of undeveloped and other problems, and achieve the effect of reversing the drug resistance of tumor cells, simple operation, and enhancing the effect of killing tumor cells
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Embodiment 1
[0041] Example 1 Preparation of cell vesicles
[0042] 1) Experimental materials and reagents
[0043] H22 mouse liver cancer cells were obtained commercially, the ultraviolet device was owned by a conventional biological safety cabinet, the transmission electron microscope was JEM1010 (JEOL, Japan), and the filter membrane brand was PALL (Part No: J100047050).
[0044] 2) Experimental steps
[0045] Cultivate H22 mouse liver cancer cells in RPMI-1640 cell culture medium to make the cell volume reach 2×10 7 / ml; H22 mouse liver cancer cells were irradiated with ultraviolet rays for 60 minutes;
[0046] Eighteen hours after ultraviolet irradiation, the H22 mouse liver cancer cell apoptosis was observed under a microscope. The cell vesicles of the above apoptotic tumor cells were collected by centrifugation, including: centrifuging at 1300rpm and 5000rpm for 10 minutes respectively, and then centrifuging at 14000g for 1 minute to remove cells and debris. The centrifugal forc...
Embodiment 2
[0049] Example 2 The effect of the combination of tumor chemotherapy drug preparations of the present invention on the efflux of doxorubicin by human breast cancer cells.
[0050] 1) Experimental materials and reagents
[0051] The human breast cancer cell line MCF-7, the drug of doxorubicin hydrochloride was purchased commercially, the ultraviolet device was owned by a conventional biological safety cabinet, and a two-photon confocal microscope was used.
[0052] 2) Experimental steps
[0053] Human breast cancer cell line MCF-7 was cultured in RPMI Medium1640 cell culture medium to make the cell volume reach 2×10 7 / ml; The human breast cancer cell line MCF-7 was irradiated with ultraviolet rays for 60 minutes; 18 hours after ultraviolet irradiation, the apoptosis of the human breast cancer cell line MCF-7 was observed under a microscope; the apoptotic tumor was collected by centrifugation or filtration Cell vesicles, the method is the same as in Example 1;
[0054] Norma...
Embodiment 3
[0058] Example 3 Effects of the combination of tumor chemotherapy drug preparations of the present invention on the uptake of doxorubicin hydrochloride by MCF-7, MCF-7 / TRC and ADR / MCF-7 cells
[0059] 1) Experimental materials and reagents
[0060] Human breast cancer cell line MCF-7, drug-resistant tumor regenerative cell MCF-7 / TRC (tumour-repopulating cancer cell), human breast cancer doxorubicin hydrochloride-resistant strain ADR / MCF-7, used for culture Three-dimensional fibrin glue, doxorubicin hydrochloride drug, lysosomal membrane protein lamp-2 antibody, FITC fluorescent secondary antibody, and nuclear dye DAPI are commercially available. The ultraviolet device is owned by a conventional biological safety cabinet, and a two-photon confocal microscope is used.
[0061] 2) Experimental steps
[0062] According to the method of Example 2, the cell vesicles derived from the human breast cancer cell line MCF-7 were obtained;
[0063] 5,000 MCF-7 human breast cancer cells w...
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