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Application of polyphosphoinositide 6-3/kinase gene in plant salt resistance

A technology of polyphosphoinositide and Arabidopsis polyphosphoinositide, which can be used in application, genetic engineering, plant genetic improvement, etc., and can solve the problem of using no Arabidopsis polyphosphoinositide 6-/3-kinase gene. Or report and other problems to overcome the impact of plant growth and yield, improve sensitivity, and maintain the effect of yield

Inactive Publication Date: 2006-10-18
WUHAN UNIV
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

No use or report of Arabidopsis polyphosphoinositide 6- / 3-kinase gene in plant salt resistance was found after searching

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  • Application of polyphosphoinositide 6-3/kinase gene in plant salt resistance
  • Application of polyphosphoinositide 6-3/kinase gene in plant salt resistance
  • Application of polyphosphoinositide 6-3/kinase gene in plant salt resistance

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Experimental program
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Embodiment 1

[0050] After the polyphosphoinositide 6- / 3-kinase gene (target gene) cloned from Arabidopsis is expressed in the present invention, the salt resistance of the transgenic Arabidopsis is obviously higher than that of the wild type. The polyphosphoinositide 6- / 3-kinase gene was assembled under the cauliflower mosaic virus 35S strong promoter (CaMV35S) with strong constitutive expression, and a vector that could be expressed in plants was constructed, and the transformation mediated by Agrobacterium The method is to make the target gene be expressed in a large amount in the transgenic plant, and the specific operation is as follows:

[0051] 1. Cloning of Arabidopsis polyphosphoinositide 6- / 3-kinase gene

[0052]Inositol 1, 4, 5-trisphosphate kinase sequences in animals were used to search for homologous sequences of inositol 1, 4, 5-trisphosphate kinase in plants in the GenBank database using Basic Local Alignment Search Tool. The polyphosphoinositide 6- / 3-kinase gene sequence f...

Embodiment 2

[0060] 1. Cloning the Arabidopsis polyphosphoinositide 6- / 3-kinase gene from Arabidopsis thaliana by the same method as in Example 1. The Arabidopsis polyphosphoinositide 6- / 3-kinase gene was inserted into the plant expression vector pBinAR-HPT to construct the pBinAR-HPT / AtIP3K expression vector. and transgenic identification. The specific method is operated as in Example 1.

[0061] 2. The screened transgenic seeds were germinated and grown at a concentration of 125mM NaCl, and compared with wild-type Arabidopsis seeds.

[0062] 3. The germination rates of transgenic and wild-type seeds were counted and the growth of seedlings was observed.

[0063] 4. Under the condition of 125mM NaCl, the individual growth of IP3K transgenic seedlings was significantly better than that of wild-type seedlings, such as image 3 Shown, the individual growth of IP3K transgenic seedlings.

[0064] 5. Under the condition of 125mM NaCl, the germination rate of IP3K transgenic seeds was also s...

Embodiment 3

[0066] 1. Cloning the Arabidopsis polyphosphoinositide 6- / 3-kinase gene from Arabidopsis thaliana by the same method as in Example 1. The Arabidopsis polyphosphoinositide 6- / 3-kinase gene was inserted into the plant expression vector pBinAR-HPT to construct the pBinAR-HPT / AtIP3K expression vector. and transgenic identification. Concrete method is identical with embodiment 1.

[0067] 2. The screened transgenic seeds were germinated and grown under 150mM and 175mM NaCl concentrations, and compared with wild-type Arabidopsis seeds.

[0068] 3. The germination rates of transgenic and wild-type seeds were counted and the growth of seedlings was observed.

[0069] 4. Under the condition of 150mM NaCl, the germination rate of IP3K transgenic seeds was also significantly higher than that of wild-type seedlings, such as Figure 5 As shown, the germination rate was increased by 48-55%, by experiment at 50%.

[0070] 5. Statistics on the germination rate of IP3K transgenic plants an...

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Abstract

The present invention discloses the apply of Arabidopsis thalianum polyphosphoinositide 6- / 3-kinase gene in plant salt resistance. Polyphosphoinositide 6- / 3-kinase gene is first cloned from target plant and then connected to corresponding vector in proper molecular biological and transgenic measures to produce transgenic plant. Applying the gene can provide one technological way for solving the problem that plant, including grain and economic crop, has low survival rate, slow growth speed and low yield in high salt environment, and is favorable to normal growth of plant in high salt area.

Description

technical field [0001] The present invention relates to the application of Arabidopsis thaliana polyphosphate 6- / 3-kinase gene (Inositol polyphosphate6- / 3-kinase) in improving the salt resistance ability of plants, more specifically to a kind of polyphosphate 6- / 3-kinase Application of kinase gene in transforming economic crops and other plants' salt tolerance. Background technique [0002] In the field of agriculture, environmental pressure is one of the important factors leading to the reduction of production. Among these environmental pressures, the harmfulness of salt stress lies in that it greatly hinders the growth of crops and ultimately reduces yield (Boyer, J.S. (1982). Plant productivity and environment. Science 218, 443-448.). Especially in irrigated areas, the salinization of land has severely limited the sustainable development of agriculture today (Ashraf, M. (1994). Breeding for salinity tolerance proteins in plants. Crit. Rev. Plant Sci. 13, 17-42 .). The ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82C12N9/16C12N15/55
Inventor 夏惠君张洪霞
Owner WUHAN UNIV
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