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Improved test system for the determination of the presence of an antibiotic in a fluid

An inspection system and antibiotic technology, applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of limited sensitivity of antibiotics and the inability to obtain sufficient application inspection systems, so as to improve sensitivity, promote accuracy, and improve vision The effect of contrast

Inactive Publication Date: 2006-08-09
DSM IP ASSETS BV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A problem with test systems currently on the market and / or described in the literature is their limited sensitivity for certain antibiotics
One of the consequences of this problem is that for some applications, for example, when threshold requirements are changed, inspection systems are not adequately available with existing technology

Method used

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  • Improved test system for the determination of the presence of an antibiotic in a fluid
  • Improved test system for the determination of the presence of an antibiotic in a fluid
  • Improved test system for the determination of the presence of an antibiotic in a fluid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Example 1 Comparison of Bromothymol Blue and Bromocresol Violet in the Determination of Penicillin G

[0050] Bromocresol Violet

bromothymol blue

Penicillin G 1

4

2

Penicillin G 2

3

2

[0051] 1. Penicillin G concentrations of 0, 2, 4 and 6 ppb were studied.

[0052] 2. Penicillin G concentrations of 0, 1, 2, 3 and 4 ppb were studied.

Embodiment 2

[0053] Example 2 Comparison of Bromothymol Blue and Bromocresol Violet in the Determination of Multiple Antibiotics

[0054] bp 1

[0055] 1.BP: bromocresol purple

Embodiment 3

[0056] Embodiment 3 The comparison of the concentration effect of bromothymol blue and bromocresol purple

[0057] Bromocresol purple (160mg l -1 )

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PUM

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Abstract

Provided is a test system, a test method and a test kit based on a test medium comprising Bromothymol Blue or a structurally related indicator.

Description

technical field [0001] The present invention relates to improved and novel microbiological test systems and novel methods of using said test systems to determine the presence of antibacterial compounds in fluids such as milk, gravy, serum and urine. Background of the invention [0002] Microbiological assay methods for the determination of antibacterial compounds, especially antibiotics (e.g. cephalosporins, penicillins, , tetracycline and its derivatives) residues, and chemotherapy drug residues, such as sulfonamides (sulfa's). Examples of such tests have been described in CA2056581, DE 3613794, EP 0005891, EP 0285792, EP0611001, GB A1467439 and US 4,946,777. Such descriptions all refer to ready-to-use tests in which microorganisms are used and the result is given by a change indicated by an indicator molecule added to the test system. The principle is that when an antibacterial compound is present in the fluid in a concentration sufficient to inhibit the growth of microo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 安吉莉娜·蒂克尔科尼利厄斯·雅各布斯·布祖基琴范·约翰尼斯·西奥多西娅·阿利亚·皮尔特德·安格里克·里克雅各布斯·斯塔克彼得·克尼里斯·朗兰
Owner DSM IP ASSETS BV
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