Decay promoting ferment and its application in composting agricultural waste
A technology of agricultural wastes and accelerators, applied in the field of decomposing accelerators, can solve the problems of difficult uniform mixing of bacteria and compost and limited application, and achieve the goals of improving composting efficiency and decomposing degree, fast fermentation temperature, and improving composting quality Effect
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[0022] The preparation of decomposing accelerator of the present invention is through following two steps:
[0023] 1. Seed culture: Scrape the purified cultured Bacillus subtilis, Aspergillus oryzae, Trichoderma reesei, Streptomyces coeruleus and purchased Phanerochaete chrysosporium from the preserved slant culture medium, and inoculate them respectively on Nutrient gravy medium, bean juice medium, Chase medium, Gao's No. 1 medium and potato sucrose medium, each 1 liter Erlenmeyer flask is filled with 200ml of liquid medium, the culture temperature is 30-37 ° C, at 150 Shake culture at ~180rpm for 24~60h.
[0024] 2. Fermentation and enzyme production culture: inoculate the above-mentioned seed culture solution into respective fermentation media with an inoculum amount of 5-8%.
[0025] The fermentation media of Bacillus subtilis, Aspergillus oryzae, Trichoderma reesei and Streptomyces coulerus have the same composition, and the formula contains (weight ratio): 2-3% of rice...
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[0035] 1. Preparation of decomposing accelerator
[0036] strain name
temperature(℃)
speed(rpm)
Culture time (h)
37
160
24
Aspergillus oryzae
35
160
24
Trichoderma reesei
30
180
40
Streptomyces coeruleus
30
180
40
37
160
48
[0037] strain name
temperature(℃)
Stirring speed (rpm)
Ventilation volume (vvm)
Culture time (d)
Bacillus subtilis
30
400
0.8
3
30
400
0.8
3
28
400
0.8
3
Streptomyces coeruleus
28
400
0.8
3
30
160
0.7
4
[0038] The cultured fermented broths were filtered to remove thalline and solid matrix and mixed according to the following proportion...
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