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Decay promoting ferment and its application in composting agricultural waste

A technology of agricultural wastes and accelerators, applied in the field of decomposing accelerators, can solve the problems of difficult uniform mixing of bacteria and compost and limited application, and achieve the goals of improving composting efficiency and decomposing degree, fast fermentation temperature, and improving composting quality Effect

Inactive Publication Date: 2006-08-02
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, there is great uncertainty in the growth of microbial agents when they are inserted into the compost system. In addition, there are a wide range of sources of compost materials, and the indigenous microbial populations will vary with different compost materials. There are certain difficulties in the dominant flora in the composting system and how to avoid the antagonism between them and the indigenous microorganisms; in addition, the inoculated bacteria and the compost are not easy to mix uniformly, which also limits the application of this method

Method used

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  • Decay promoting ferment and its application in composting agricultural waste
  • Decay promoting ferment and its application in composting agricultural waste

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preparation example Construction

[0022] The preparation of decomposing accelerator of the present invention is through following two steps:

[0023] 1. Seed culture: Scrape the purified cultured Bacillus subtilis, Aspergillus oryzae, Trichoderma reesei, Streptomyces coeruleus and purchased Phanerochaete chrysosporium from the preserved slant culture medium, and inoculate them respectively on Nutrient gravy medium, bean juice medium, Chase medium, Gao's No. 1 medium and potato sucrose medium, each 1 liter Erlenmeyer flask is filled with 200ml of liquid medium, the culture temperature is 30-37 ° C, at 150 Shake culture at ~180rpm for 24~60h.

[0024] 2. Fermentation and enzyme production culture: inoculate the above-mentioned seed culture solution into respective fermentation media with an inoculum amount of 5-8%.

[0025] The fermentation media of Bacillus subtilis, Aspergillus oryzae, Trichoderma reesei and Streptomyces coulerus have the same composition, and the formula contains (weight ratio): 2-3% of rice...

Embodiment

[0035] 1. Preparation of decomposing accelerator

[0036] strain name

temperature(℃)

speed(rpm)

Culture time (h)

Bacillus subtilis

37

160

24

Aspergillus oryzae

35

160

24

Trichoderma reesei

30

180

40

Streptomyces coeruleus

30

180

40

Phanerochaete chrysosporium

37

160

48

[0037] strain name

temperature(℃)

Stirring speed (rpm)

Ventilation volume (vvm)

Culture time (d)

Bacillus subtilis

30

400

0.8

3

Aspergillus oryzae

30

400

0.8

3

Trichoderma reesei

28

400

0.8

3

Streptomyces coeruleus

28

400

0.8

3

Phanerochaete chrysosporium

30

160

0.7

4

[0038] The cultured fermented broths were filtered to remove thalline and solid matrix and mixed according to the following proportion...

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Abstract

The present invention is decay promoting ferment and its application in composting agricultural waste to promote the degradation of hemicellulose, cellulose and lignin. The decay promoting ferment is one compound fermented liquid compounded with the fermented liquid of starch decomposing bacteria, pectin decomposing bacteria, hemicellulose decomposing bacteria, cellulose decomposing bacteria and lignin decomposing bacteria, and through filtering off thallus and mixing. It is added into and mixed with the compost material, with the added amount being 0.8-1.5 wt% of the compost material. It can promote the degradation of starch and other easy-to-degrade matters as well as pectin, lignin and cellulose. It has fast fermentation temperature raising and simple use, and can promote composting efficiency and improve compost quality.

Description

technical field [0001] The invention relates to a decomposing accelerator, in particular to a composite fermented liquid prepared by mixing crude enzyme liquids fermented by different microorganisms and its application in composting agricultural waste. Background technique [0002] my country produces more than 700 million tons of crop straw every year, and most of these materials are directly invaded into the environment as agricultural waste in the form of accumulation and burning, causing great environmental pollution and waste of resources. Aerobic composting is an important means of resourceful and harmless treatment of agricultural waste. Traditional aerobic composting mainly uses the indigenous microorganisms in the compost to metabolize and decompose organic matter, but since the cell walls of plant tissues in agricultural waste are mainly composed of pectin, cellulose, hemicellulose and lignin, the structure Stable and not easy to degrade, especially the refractory...

Claims

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Application Information

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IPC IPC(8): C05F17/00C12N1/00
CPCY02W30/40
Inventor 黄丹莲曾光明冯冲凌蒋晓云刘鸿亮
Owner HUNAN UNIV
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