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Idesia polycarpa Idesia tissue culture method

A technique of tissue culture and mountain papaya, applied in horticultural methods, botany equipment and methods, plant cells, etc., can solve the problems of mixed male and female seedlings, low germination rate of seeds, and indistinguishable sexes

Inactive Publication Date: 2006-07-19
JIANGSU ACAD OF FORESTRY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, there is no report on the tissue culture method of Jacarina, and the existing propagation method of Jatropha is to propagate with seeds. Because the germination rate of seeds is low, the quality of seedlings is uneven, and the requirements for good seedlings cannot be reached; at the same time, the tree species It is dioecious, and the fruit of the female plant has more ornamental value, but the seedlings are mixed with male and female, and it is difficult to distinguish between male and female. Through the method of tissue culture, female plants can be selected for mass reproduction.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Tissue Culture of Jacarna

[0021] The basic culture medium used is as follows (substance type and quality contained in each liter):

[0022] Potassium nitrate 600ml, ammonium nitrate 500mg, calcium chloride dihydrate 220ml, magnesium sulfate heptahydrate 200mg, potassium dihydrogen phosphate 75mg, disodium edetate 49.8mg, ferrous sulfate 37.1mg, tetrahydrate Manganese sulfate 12 mg, zinc sulfate heptahydrate 4.5 mg, boric acid 6.2 mg, potassium iodide 0.42 mg, sodium molybdate dihydrate 0.25 mg, copper sulfate pentahydrate 0.025 mg, cobalt chloride hexahydrate 0.025 mg, thiamine hydrochloride 0.18 mg, Niacin 1.0 mg, pyridoxine hydrochloride 0.5 mg, inositol 80 mg, glycine 2 mg, sucrose 20 mg, carrageenan 6.5 g, and finally adjust the pH to 5.8-6.0.

[0023] The inoculation and starting medium are: the above-mentioned basic medium is supplemented with TDZ 0.02+BA 1.0+NAA 0.05 (mg / L)

[0024] Proliferation medium: add TDZ 0.02+BA 1.5+NAA 0.1 (mg / L) to the ba...

Embodiment 2

[0034] Embodiment 2. The tissue culture of jatropha

[0035] The basic culture medium used is as follows (substance type and quality contained in each liter):

[0036] Potassium nitrate 800ml, ammonium nitrate 650mg, calcium chloride dihydrate 330ml, magnesium sulfate heptahydrate 370mg, potassium dihydrogen phosphate 90mg, disodium edetate 49.8mg, ferrous sulfate 37.1mg, tetrahydrate Manganese sulfate 12 mg, zinc sulfate heptahydrate 4.5 mg, boric acid 6.2 mg, potassium iodide 0.42 mg, sodium molybdate dihydrate 0.25 mg, copper sulfate pentahydrate 0.025 mg, cobalt chloride hexahydrate 0.025 mg, thiamine hydrochloride 0.18 mg, Niacin 1.0 mg, pyridoxine hydrochloride 0.5 mg, inositol 80 mg, glycine 2 mg, sucrose 20 mg, carrageenan 6.5 g, and finally adjust the pH to 5.8-6.0.

[0037] Inoculation and starting medium are: the above basic medium is added with TDZ 0.05+BA 1.2+NAA 0.2 (mg / L),

[0038] The proliferation medium is: the above basic medium is supplemented with TDZ 0.08...

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PUM

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Abstract

A tissue culture process for mountain tung, essentially comprising the following steps: 1. sterilizing the culture medium under high temperature and pressure, 2. taking the auxiliary bud stem of good seed quality and plant quality as explant, sterilizing with alcohol and corrosive mercury chloride solution, washing with sterilized water, 3. pitching the sterilized explant into culture medium, and putting under artificial daylight lamp for differentiation and bud growth, 4. cutting bud and pitching it into enrichment culture medium, 5. cutting the tube bud and pitching it into sprout-strengthening culture medium, 6. removing the base callus and part of leaves of the tube strengthened sprout, leaving 3-5 leaves and pitching them into root-growing culture medium, 7. taking out the sprout with root for washing, replanting it to culture medium with chaff ash and brown soil, watering thoroughly, shading for 70% in the first 10 days, and then lighting gradually, 20 days later the sprout survivals with root growing out rate being above 85%, and totally lighting 35-45 days later.

Description

Technical field: [0001] The invention relates to a plant tissue culture method, in particular to a tissue culture method of Idesia polycarpa Maxim. Background technique: [0002] In the prior art, there is no report on the tissue culture method of Jacarina, and the existing propagation method of Jatropha is to propagate with seeds. Because the germination rate of seeds is low, the quality of seedlings is uneven, and the requirements for good seedlings cannot be reached; at the same time, the tree species It is dioecious, and the fruits of female plants have more ornamental value, but the male and female plants are mixed in sowing seedlings, and it is difficult to distinguish between male and female. Through the method of tissue culture, female plants can be selected for mass reproduction. Invention content: [0003] The object of the present invention is to provide a method for tissue culture of jacaranda. [0004] The technical scheme of the present invention is as follo...

Claims

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Application Information

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IPC IPC(8): A01H4/00C12N5/04
Inventor 蒋泽平梁珍海吴纲季永华刘根林朱学雷李玉巧李劲李淑琴杨勇仇曙光袁芳
Owner JIANGSU ACAD OF FORESTRY
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