Non-tube quick reproduction method for seedings of jatropha curcas L.
A tung tree and fast technology, applied in horticultural methods, botanical equipment and methods, plant cells, etc., can solve the problems of high production cost, difficulty in meeting the demand for quantity and quality of tung tree seedlings, low survival rate, etc. The effect of saving germplasm material, improving the efficiency of rapid propagation and large amount of material
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[0026] Both the embodiment and the comparative example adopt 1-year-old tung tree branches to intercept the micro-stem section for rapid propagation.
[0027]Sterilization treatment: Remove the leaves from the harvested branches, soak them in disinfectant [5% sodium hypochlorite (NaClO), 0.1% Tween-20] for 20 minutes, and then rinse them with tap water 5 times; cut the branches into shorter ones of about 15-20 cm Stem segments were soaked in 70% ethanol for 10 minutes, then soaked in disinfectant solution for 15 minutes, and rinsed with sterile water for 5 times to thoroughly wash away residual sodium hypochlorite.
[0028] Prepare cytologin indole butyric acid (IBA) solution; the concentrations are 0.2, 0.5, 1.0, 2.0 (mg / L).
[0029] Prepare nutrient solution: prepare 1 / 2 MS basic medium, pH 5.8-6.0, see Table 1 for specific formula.
[0030] Element
[0031] Non-test tube rapid propagation: cut the sterilized stems into 2-3cm long micro-stems under aseptic co...
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