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Method for quickly breeding Akebia trifoliata Koidz fruit seedling in test tube

A technology of three-leaf clover and seedlings, which is applied in horticultural methods, botanical equipment and methods, horticulture and other directions, can solve the problems of low survival rate, difficult rooting, slow reproduction and the like

Inactive Publication Date: 2011-06-15
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

[0003] The production of excellent seedlings of Akebia trifoliata generally adopts the cutting method, but there are problems such as rooting difficulty, low survival rate, and slow propagation speed. Cell engineering technology can be used to multiply excellent Akebia trifoliate seedlings in a large number in a short period of time, which can not only It is a good solution to the difficult problem of breeding Akebia trifoliate seedlings, and it is also the premise and basis for improving the traits of Akebia trifoliata by using cytogenetic engineering technology

Method used

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Embodiment Construction

[0022] A New Method of Improving Rapid Propagation of Akebia Trifoliata Seedlings in Test Tube

[0023] (1) Plant the fine seedlings of Akebia trifoliata collected from Zhangjiajie, Hunan, Huaihua, Henan, and Luoyang, Henan in the greenhouse. When the long branches of 30-40cm grow from the Akebia trifoliata seedlings, select strong branches, which will bring Part of the stems of the axillary buds are cut off and rinsed with tap water. The stem segment with axillary buds was used as explant, after being sterilized with 75% alcohol for 30 seconds, then sterilized with 0.1% mercuric chloride for 8 minutes, then rinsed with sterile water for 4 to 5 times, cut with a scalpel after drying, and made the The length of the axillary bud part is about 1 cm, and they are respectively inserted on the cluster bud induction medium according to the natural growth direction of the stem, and 5 explants are inoculated in each triangular flask, about 20 flasks are inoculated with each explant, 25...

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Abstract

The invention relates to a method for quickly breeding an Akebia trifoliata Koidz fruit seedling in a test tube, comprising the following steps of: inducing Akebia trifoliata Koidz to generate cluster buds, placing the cluster buds into a culture medium for rooting and culturing; then culturing a plant to the height of about 7cm at the temperature of 25 + / - 2 DEG C in an illumination environment; taking out the cluster buds from the culture medium; cutting the seedling in the test tube with a scalpel into stem sections with axillary buds of about 1cm for rooting and culturing; and then illuminating the plant to grow into about 7cm again, i.e. illuminating again and transplanting the plant to a cultivation substrate for growing. By adopting a genetic engineering technology of plant cells, the method can greatly shorten the breeding time, quicken the breeding progress and obtain a new species with more ideal characteristics purposefully.

Description

technical field [0001] The invention relates to a new method for improving the technical field of rapid propagation of Akebia trifoliata seedlings, in particular to a treatment method for explants. Background technique [0002] Akebia trifoliata (Thunb.) Koidz] is a deciduous or semi-deciduous vine wild fruit with high nutritional value in the genus Akebia. It is mostly distributed in the south of the Yangtze River, in southern China, the Yangtze River Basin and It is distributed in Northwest China and is known as the king of wild fruits together with kiwi, commonly known as August Fried and August Melon. For a long time, people have regarded the roots, stems, leaves, and seeds of the Akebia family as Chinese herbal medicines, which have the functions of clearing tendons and collaterals, detoxifying, promoting body fluids and quenching thirst, reducing inflammation, dehumidifying, purging fire, and clearing milk. Its fruit is relatively large, weighing 150-250g, kidney-shap...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 艾辛范立华
Owner HUNAN AGRICULTURAL UNIV
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