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Transgenic soybean detection method and the primer

A technology of genetically modified soybeans and detection methods, which is applied to the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as inability to adapt, time-consuming and labor-consuming, and long-term problems, so as to reduce detection costs, improve detection efficiency, and improve The effect of work efficiency

Inactive Publication Date: 2006-05-10
TIANJIN NORMAL UNIVERSITY
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Problems solved by technology

More importantly, the current detection methods for genetically modified soybeans generally take a long time, are time-consuming, labor-intensive, and expensive, and cannot meet the needs of rapid response to market changes, which is not conducive to the international competition of domestic soybean processing and trading companies

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  • Transgenic soybean detection method and the primer
  • Transgenic soybean detection method and the primer
  • Transgenic soybean detection method and the primer

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Embodiment 1

[0037] The transgenic soybeans (Z) used in the embodiments of the present invention are imported from the United States, and the non-transgenic soybeans are provided by Tianjin Academy of Agricultural Sciences, which are respectively: Northeast soybeans (D), Northeast green soybeans (Dq) and Hebei soybeans (H) (described Z, D, Dq, and H represent the four varieties respectively, see figure 1 ).

[0038] The used instrument of the embodiment of the present invention mainly comprises: PCR (polymerase chain reaction, polymerase chain reaction) instrument (GeneAmp PCR System 9700); DNA electrophoresis instrument (Beijing Liuyi, DYY-III 31B); Ultraviolet transmission reflection analyzer (ZF type, Shanghai Kanghua Biochemical Instrument Factory); UVI gel imaging system (Mitsubishi, BTS-20-M).

[0039] The detection method of transgenic soybean of the present invention comprises:

[0040] 1. Extract soybean DNA.

[0041] Large DNA was extracted by phenolform extraction. The specifi...

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Abstract

This invention relates to a transfer gene soybean checking method and its eduction, which includes:1 distilling soybean DNA;2 PCR expanding reaction, the total volume of the system is 25ul, each component and its final concentration is as follows: cushion solution 10mM, dNTP0.2mMú¼Mg2+1.5mMú¼the up eduction and the down eduction are 0.2mMú¼Taq 1Uú¼DNA50ng respectively, the special eduction react under the condition of the special PCR reaction by filling ddH20 up to the 25ul; after the reaction ending, distills the systerm solution 10ul and makes electrophoresis detection with 2.0úÑ agarose gelatin, and makes record; 3judging whether the soybean is the transfer gene soybean according to the record that whether it can display a 337bp unusual zoster. The related matter includes the up eduction and the down matter, whichare:Fú‘5'-CACTGACGTAAGGGATGA-3 18nt Tmú‘55.0ú¼Rú‘5'-TGTGCTGTAGCCACTGAT-3'18nt Tmú‘55.0.

Description

technical field [0001] The invention relates to a detection method for transgenic soybean products, specifically a method for amplifying the CaMV35s promoter sequence and EPSPS sequence used for transgenic soybeans by designing primers, and determining whether it is a transgenic soybean according to whether a specific fragment can be amplified The detection method of soybean and the primers used, the main international patent classification number is proposed to be Int.CL 7 C12Q 1 / 68. Background technique [0002] China is the fourth soybean producer in the world, with an annual output of 15-18 million tons, all of which are traditional non-GMO soybeans. But every year also need to import a large amount of soybeans from abroad. Almost 100% of imported soybeans are genetically modified soybeans. According to reports, the oil yield of genetically modified soybeans can reach 20-21%. In contrast, the oil yield of domestic soybeans is only 17%, but the price is higher than th...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 王景安武泰存梁晓华
Owner TIANJIN NORMAL UNIVERSITY
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