Eukaryon expression of coli heat-sensitive toxin B subunit
A technology of Escherichia coli and an expression method, which is applied in the field of eukaryotic expression of Escherichia coli heat-sensitive toxin B subunit, and achieves the effects of less interference factors, strong specificity, convenience and quickness of specificity
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment
[0026] 1. Cloning and sequencing of LT-B gene with signal peptide sequence
[0027] Cloned from pathogenic Escherichia coli E.coli O6:H16 (LT) inserted into the pGEM-T vector + , ST + ) LTB whole gene as a template, with primers Pb1: 5'-ATG AAT AAA GTA AAA TGT TAT-3 and Pb2: 5'-GAA TTC TAG TTT TCCATA CTG ATT GCC-3, the signal peptide was amplified by high-fidelity PCR The LT-B sequence (sequence attached below) was subcloned into pGEM-T, and the LT-B with the signal peptide sequence was correct after sequencing. Its sequence is as follows, with a total of 372bp, which can encode 103 amino acids of the mature peptide LT-B.
[0028] 1 atgaataaag taaaatgtta tgttttatt acggcgttac tatcctctct atgtgcatac
[0029] 71 ggagctcccc agtctattac agaactatgt tcggaatatc gcaacacaca aatatatacg
[0030] 121 ataaatgaca agatactatc atatacggaa tcgatggcag gtaaaagaga aatggttatc
[0031] 181 attacattta agagcggcgc aacatttcag gtcgaagtcc cgggcagtca acatatagac
[0032] 241 tcccaaaaaa aagccattga aaggatgaa...
PUM
Property | Measurement | Unit |
---|---|---|
molecular weight | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com