Preparation type electrochromatography separation arrangement with applied electric field being vertical to flow direction

Abstract

The invention relates to a preparative electrochromatogram separation device, belonging to the field of process and separation technique of biological product. The device is a triple chamber rectangle electro chromatographic column, which comprises a gel chamber loaded with filler in the middle and electrode chambers at double- side, the gel chamber 8 being a hollow plexiglass body with symmetrical trenches at the double sides and the middle being rectangle; the trench being equipped with diaphragm 3 which can intercept the molecular of a molecular weight more than 1000 Dalton and can let conductive ion transit freely; two electrode chambers 9 which is equipped with inert platinum electrodes 5 being at double- outside of gel chamber; and the electrode chambers 9 and gel chamber 8 being loaded with acetate, phosphonate, hydrogen carbonate or trishydroxymethylaminomethane- hydrochloric acid solution. The device invented is characterized in that the separating power is strong, the handling capacity is large, the applicability extensive, and it has an extensive application prospect in separating and purifying the biomacromolecule such as protein and nucleic acid.

Description

technical field [0001] The invention relates to a preparative electrochromatographic separation device for separating biological macromolecules such as proteins and applying an electric field perpendicular to the flow direction, belonging to the technical field of biological product processing and separation. Background technique [0002] In the amplification process of preparative electrochromatography, in addition to solving the problem of introducing a stable electric field in the chromatography, many problems caused by the introduction of an external electric field must be solved. One of the most important is to solve the problem of Joule heating and electrolysis products. Most proteins will denature rapidly at about 40°C. The generation of Joule heat in electrochromatography is proportional to the square of the column cross-sectional area and voltage. When electrochromatography is amplified, on the one hand, increasing the column heigh...

AI Technical Summary

Problems solved by technology

However, it still has the disadvantages of axial electric field electrochromatography, that is, the distance between the two electrodes of axial electric field electrochromatography is long, and the applied voltage is high, resulting in serious Joule heating effect in the column and large cooling load; during operation, the continuous flow of positive and negative ions Directional migration will still cause an increase in the opposite charge at the electrode, resulting in a decrease in the electric field intensity in the column

At the same time, a large amount of protein will be adsorbed on the separator of the opposite electrode, resulting in product loss

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