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Method for producing gamma-propalanine using saliva chain coccus thermophilous subspecies

A technology of Streptococcus salivarius and aminobutyric acid, which is applied in the field of biosynthesis of gamma-aminobutyric acid, can solve the problems of gamma-aminobutyric acid that have not been seen, and achieves the effects of high safety and reduced production costs

Inactive Publication Date: 2005-12-21
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the use of Streptococcus salivarius subsp. thermophile or its glutamic acid decarboxylase to produce γ-aminobutyric acid

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Streptococcus salivarius thermophilic subspecies is through expanded culture, centrifuges and collects thalline, with reference to " enzyme engineering " (Guo Yong chief editor, China Light Industry Press, 2000) described method is to the glutamic acid of streptococcus salivarius thermophilic subspecies Decarboxylase for extraction. Then mix the enzyme solution with glutamic acid or glutamate solution, and carry out stirring reaction at pH 3.2-8.0, 20-80°C for 1h-3d to obtain γ-aminobutyric acid containing 0.1%-70% The reaction solution.

Embodiment 2

[0018] Streptococcus salivarius thermophilic subspecies is through expanded culture, and centrifugation collects thalline, and thalline is broken up, and with reference to " enzyme engineering " (Guo Yong chief editor, China Light Industry Press, 2000) described method to Streptococcus salivarius thermophilic Subspecies of glutamic acid decarboxylase were extracted. Then mix the enzyme liquid with the substance containing glutamic acid or glutamate, adjust the pH to 3.2-8.0, and react at 20-80°C for 1-5 days to obtain the base material containing γ-aminobutyric acid.

Embodiment 3

[0020] After the expanded culture of Streptococcus salivarius thermophilic subspecies, the bacteria are collected by centrifugation, and then the bacteria are mixed with glutamic acid or glutamate solution, and the stirring reaction is carried out at pH 3.2-8.0, 20-80°C for 1- On the 10th day, the bacterial cells were separated by centrifugation to obtain transformation liquids with different contents of γ-aminobutyric acid.

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PUM

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Abstract

the invention explains a gamma - amino butyric acid's production method, which uses Streptococcus thermophilus as the bacterial acting on glutaminic acid , glutaminic acid salt , material containing glutaminic acid or glutaminic acid salt to make alpha - he carboxyl of glutaminic acid take decarboxylation reaction , thus turn into gamma - amino butyric acid. This method is a biological synthetic method, does not need high-temperature high pressure reaction condition and harmful raw materials, the uniqueness is high, accessory substances is little and safety is high.

Description

technical field [0001] The invention relates to a method for producing gamma-aminobutyric acid by using Streptococcus salivarius subsp. Production of gamma-aminobutyric acid by glutamate decarboxylase from Bacillus subsp. Background technique [0002] γ-Aminobutyric acid (γ-Aminobutyric acid, GABA), also known as aminobutyric acid, is a natural amino acid composed of non-protein, which is a major inhibitory neurotransmitter in the mammalian central nervous system and has important physiological functions , such as lowering blood pressure, diuresis, analgesia, improving brain function, enhancing brain vitality, promoting long-term memory, nourishing nerve cells, improving menopausal syndrome, etc. Long-term lack of GABA in the brain will lead to epilepsy, Parkinson and other diseases. At the same time, GABA is also related to brain aging, and its deficiency will lead to "deafness and blindness" in the elderly. In addition, GABA can promote the ability of sperm to penetrate...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00
Inventor 陆兆新杨胜远吕凤霞别小姝邹晓葵曾晓雄
Owner NANJING AGRICULTURAL UNIVERSITY
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