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Method for biologically synthesizing gamma-aminobutyric acid by pediococcus pentosaceus

A technology of Pediococcus pentosaceae and aminobutyric acid, applied in the biological field, can solve the problems that have not yet been seen in the synthesis of γ-aminobutyric acid, and achieve the effect of less waste, less by-products, less material and energy

Inactive Publication Date: 2011-11-16
HANSHAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Pediococcus pentosaceus has been widely used in food and feed, there is no report on the biosynthesis of GABA by Pediococcus pentosaceus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Pediococcus pentosaceae was expanded and cultivated through MRS, potato juice compound proliferation medium, wort juice compound proliferation medium or bean sprouts juice compound proliferation medium, and the bacterial cells were collected by centrifugation, referring to "Enzyme Engineering" (Edited by Guo Yong, China Light Industry Press , 2000) described method extracts the glutamate decarboxylase of Pediococcus pentosaceus. Then mix the enzyme solution with glutamic acid or sodium glutamate solution, and carry out stirring reaction at pH 3.2-5.5, 20-45°C for 1h-3d to obtain γ-aminobutyric acid with a concentration of 1%-20%. solution.

Embodiment 2

[0053] After expanded culture of Pediococcus pentosaceae through MRS, potato juice compound proliferation medium, wort juice compound proliferation medium or bean sprouts juice compound proliferation medium, the bacteria were collected by centrifugation, and then the bacteria were mixed with glutamic acid or sodium glutamate solution Mix, carry out stirring reaction at pH 3.2-5.5, 20-45° C. for 1-4 days, and centrifuge to separate the bacterium to obtain a γ-aminobutyric acid solution with a concentration of 1%-30%.

Embodiment 3

[0055] After expanded culture of Pediococcus pentosaceae through MRS, potato juice compound proliferation medium, wort juice compound proliferation medium or bean sprouts juice compound proliferation medium, the bacteria were collected by centrifugation, and then the bacteria were mixed with glutamic acid or sodium glutamate solution Mix, add an appropriate amount of surfactants such as Tween, sucrose ester or Triton, carry out stirring reaction at pH 3.2-5.5, 20-45°C for 1-4 days, and centrifuge to separate the bacteria. A gamma-aminobutyric acid solution with a concentration of 1% to 30% is obtained.

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Abstract

The invention discloses a method for biologically synthesizing gamma-aminobutyric acid, which uses the pediococcus pentosaceus as the strain, and converts the externally added glutamic acid or monosodium glutamate through fermentation engineering technology, cell transformation method and enzyme engineering technology to produce high content gamma-aminobutyric acid (conversion liquid gamma-aminobutyric acid content reaches 1-30%). The method belongs to the biological technical field, doesn't need high temperature, high pressure reaction condition or toxic harmful raw materials, has the advantages of high maneuverability and little by-product, and the pediococcus pentosaceus is a food grade microorganism with high safety, thus applying to industries such as chemical industry, food, forage or medicine after purifying.

Description

technical field [0001] The invention relates to a method for synthesizing gamma-aminobutyric acid by using microorganisms, in particular to a method for synthesizing gamma-aminobutyric acid by using Pediococcus pentosaceus, and belongs to the field of biotechnology. Background technique [0002] γ-Aminobutyric acid (γ-Aminobutyric acid, GABA), also known as aminobutyric acid, is a natural amino acid that is not composed of proteins. It is a major inhibitory neurotransmitter in the mammalian central nervous system and has important physiological functions. , such as lowering blood pressure, diuresis, analgesia, improving brain function, enhancing brain vitality, promoting long-term memory, nourishing nerve cells, improving menopausal syndrome, etc. Long-term lack of GABA in the brain will lead to epilepsy, Parkinson and other diseases. At the same time, GABA is also related to brain aging, and its deficiency will lead to "deafness and blindness" in the elderly. In addition,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P13/00C12R1/01
Inventor 杨胜远李云韦锦
Owner HANSHAN NORMAL UNIV
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