Flat wire having indication wire and its insertion method

Abstract

RNA interference (RNAi) has become a powerful tool for blocking gene expression in mammals and mammalian cells. This approach requires the delivery of small interfering RNA (siRNA) either as RNA itself or as DNA, using an expression plasmid or virus and the coding sequence for small hairpin RNAs that are processed to siRNAs. Current expression systems for the production of siRNA in vivo rely on RNA polymerase III promoters. These are difficult to regulate and leave most of the RNA in the nucleus of the cell where it is inactive for RNA interference. The invention provides a DNA cassette for the cloning of small hairpin sequences which permit their expression and processing using RNA polymerase II. This system enables efficient transport of the pre-siRNAs to the cytoplasm where they are active and permits the use of regulated and tissue specific promoters for gene expression.

Description

technical field [0001] The invention relates to a connection object, in particular to a flat cable and its insertion method. Background technique [0002] Flexible flat cables are usually used in electronic devices as a connection structure, and the transmission lines in the flexible flat cables are wrapped in polyester film shielding. Flexible cable can be used in a small place for effective connection. For example, a removable device can be connected to a printed circuit board through flexible cables. Flexible flex cables are used in many consumer electronic devices, such as scanners, liquid crystal displays, optical storage devices, and similar devices. [0003] Figure 1a is a schematic representation of an existing flat line perspective. A flat cable 11 includes a plurality of transmission lines 12a to 12f, and a flexible plastic insulating layer 15 covering the transmission lines 12a to 12f. And the ends of the transmission lines, such as 13a to 13f, are exposed on...

AI Technical Summary

Problems solved by technology

Otherwise, an electronic device may fail if the flat wire is not inserted far enough

In order to overcome the above disadvantages, a new flat wire and operation method are needed to avoid insufficient insertion distance of the flat wire

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