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Reagent preparing method for rapidly detecting blood ketone body

A technology of blood ketones and reagents, applied in the field of biosensors, can solve the problems of unpopularity of β-hydroxybutyric acid

Inactive Publication Date: 2005-10-05
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, enzymatic determination of β-hydroxybutyric acid is not popular in China, and a new rapid detection method for blood ketones is urgently needed

Method used

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  • Reagent preparing method for rapidly detecting blood ketone body
  • Reagent preparing method for rapidly detecting blood ketone body
  • Reagent preparing method for rapidly detecting blood ketone body

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0033] Example 1: For the scanning electron microscope (SEM) images of metal nanoparticles assembled on the electrode and the cyclic voltammetry curves before and after the metal nanoparticles are assembled on the electrode, please refer to the patent application No. 03141154.1.

[0034] The experiment adopts chronoamperometry, connects the electrode to be plated to the working electrode of the CHI electrochemical workstation, the platinum electrode is used as the counter electrode, and the reference electrode of the system is shorted to the counter electrode. response curve as figure 2 As shown, the electrolyte is composed of 48mM chloroplatinic acid and 5mM lead acetate solution, the electroplating time is 300s, and the current is quickly stabilized at about 1A. After electrodeposition, rinse the electrode surface with a large amount of deionized water to remove various ions remaining on the electrode surface, dry it in an electric blast drying oven at 37°C for 10 minutes, ...

Embodiment 2

[0036]Example 2: Take the test strip that has been modified with metal nanoparticles, add 2ul of the reaction reagent containing β-hydroxybutyrate dehydrogenase dropwise on the nanoparticle layer, and then place it in an electric blast drying oven at 37°C Dry for 30 minutes, take it out; then add 5ul of a ketone body solution with a concentration of 4mM β-hydroxybutyric acid dropwise, and use a CHI electrochemical workstation for detection. The reference electrode and the counter electrode of the system are short-circuited, and the working voltage here is -0.2V ,Such as image 3 Time-response curves for ketone bodies.

[0037] The formed reaction reagents include 5mg / ml β-hydroxybutyrate dehydrogenase; 3wt% Triton X-100; 10mM coenzyme; 6mg / ml diaphorase; 50mM ferricyanate;

[0038] Figure 4 Represents the ketone body (β-hydroxybutyric acid) solution response calibration curve. Among them, the test strip is prepared by micro-electromechanical system (MEMS) technology (CV<5%...

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Abstract

The invention discloses a reagent preparation technique which can fast test the blood ketone. The technique includes first assemble a level of metal nanometer particles on pre-processed electric pole with electrochemical deposit technique. And then fix the reaction reagent on the nanometer particle layer forming reaction layer. When blood ketone drop is dropt on the reaction layer, the basal material will reaction with the solution generate electric signals testable with electrochemistry bio sensor testing system outputting corresponding intensity signal.

Description

technical field [0001] The invention relates to the technical field of biosensors, and relates to a preparation method of a reagent for rapid detection of blood ketone bodies by an electric current type biosensor. The test strip can rapidly detect the concentration of β-hydroxybutyric acid in blood ketone bodies. Background technique [0002] Ketone bodies are the unique intermediate metabolites of fatty acids in the liver when they are decomposed and oxidized. They include acetoacetate, β-hydroxybutyrate and acetone, which are the general designation of the three. And β-hydroxybutyric acid is the main component of blood ketone bodies, accounting for about 78% of ketone bodies. When the metabolism of carbohydrates in the body is disturbed, and the synthesis exceeds the decomposition, the level of blood ketone bodies increases, or when inappropriate excessive transfusion of long-chain fat emulsion is used as energy, the level of blood ketone bodies will also increase, causing...

Claims

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Application Information

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IPC IPC(8): G01N27/413G01N33/66
Inventor 姜利英蔡新霞李华清刘春秀罗贤波吴一戎
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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