Vitrified freeze keeping liquid for biological tissue cell

Abstract

This invention relates to a composition material, namely a glassivation freeze-storing liquid, for maintaining active of biological tissue cell stored in profound hypothermia, which makes up present technology deficiency to supply a glassivation freeze-storing liquid suitable for various tissue cells to maintain their active. The invention comprises the following compositions with its percentage by volume: 19.5-21.5 dimethyl sulfoxide, 14.5-16.5 acetamide, 3.5-5.5 propanediol, 4.5-6.5 2, 3-butanediol and 5-7 carbowax with its molecular weight being 6000. The invention has the advantages of allocation simplicity, operation convenience, lower price.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a composition capable of maintaining the activity of cryopreserved biological tissue cells. Further, it relates to a vitrification solution for cryopreserving biological tissue cells. Background technique [0002] At present, the conventional method for deep cryopreservation of biological tissue cells is the slow freezing method. This method generally needs to be equipped with a cryopreservation solution and a programmed cooling device, and the operation process is relatively complicated. Take the deep cryopreservation of rat embryonic nerve cells as an example: the cryopreservation medium used is RPMI 1640 solution (culture medium) containing 20% ​​DMSO (dimethyl sulfoxide) and 40% calf serum. The cell suspension was mixed in equal amounts so that the final concentration of DMSO was 10%. After equilibrating at 4°C for 20 minutes, the temperature was controlled with a temperature pro...

AI Technical Summary

Problems solved by technology

[0003] Due to the high price of the programmed cooling instrument equipped with the slow freezing method, general-scale application units are unable to purchase it; the programmed cooling instrument is large in size and inconvenient to carry. It is particularly difficult to use; and when the program cooling device is used, the processing time is longer due to the slow cooling, which generally takes more than an hour.

Due to the above-mentioned many defects in the slow freezing method, its application has been greatly limited.