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Vitrified freeze keeping liquid for biological tissue cell

A technology of vitrification and cryopreservation of biological tissues, applied in biochemical equipment and methods, microorganisms, tissue culture, etc., can solve the problems of long processing time, difficult use, and inconvenient portability, and achieve simplified cryopreservation procedures and easy promotion and use , the effect of improving the survival rate

Inactive Publication Date: 2005-09-21
胡军祥 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the high price of the programmed cooling instrument equipped with the slow freezing method, general-scale application units are unable to purchase it; the programmed cooling instrument is large in size and inconvenient to carry. It is particularly difficult to use; and when the program cooling device is used, the processing time is longer due to the slow cooling, which generally takes more than an hour.
Due to the above-mentioned many defects in the slow freezing method, its application has been greatly limited.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] The vitrification solution in Example 1 contains: (volume percentage) DMSO-19.5, acetamide-14.5, propylene glycol-3.5, 2,3-butanediol-4.5, polyethylene glycol-5.

Embodiment 2

[0016] The vitrification solution in Example 2 contains: (volume percent) DMSO-21.5, acetamide-16.5, propylene glycol-5.5, 2,3-butanediol-6.5, polyethylene glycol-7.

Embodiment 3

[0017] The vitrification solution in Example 3 contains: (volume percent) DMSO-20.5, acetamide-15.5, propylene glycol-4.5, 2,3-butanediol-5.5, polyethylene glycol-6.

[0018] Two, the aforementioned embodiment is tested in the following manner simultaneously:

[0019] 1. The effect of vitrification solution on the recovery rate of embryonic nerve cells

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PUM

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Abstract

This invention relates to a composition material, namely a glassivation freeze-storing liquid, for maintaining active of biological tissue cell stored in profound hypothermia, which makes up present technology deficiency to supply a glassivation freeze-storing liquid suitable for various tissue cells to maintain their active. The invention comprises the following compositions with its percentage by volume: 19.5-21.5 dimethyl sulfoxide, 14.5-16.5 acetamide, 3.5-5.5 propanediol, 4.5-6.5 2, 3-butanediol and 5-7 carbowax with its molecular weight being 6000. The invention has the advantages of allocation simplicity, operation convenience, lower price.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a composition capable of maintaining the activity of cryopreserved biological tissue cells. Further, it relates to a vitrification solution for cryopreserving biological tissue cells. Background technique [0002] At present, the conventional method for deep cryopreservation of biological tissue cells is the slow freezing method. This method generally needs to be equipped with a cryopreservation solution and a programmed cooling device, and the operation process is relatively complicated. Take the deep cryopreservation of rat embryonic nerve cells as an example: the cryopreservation medium used is RPMI 1640 solution (culture medium) containing 20% ​​DMSO (dimethyl sulfoxide) and 40% calf serum. The cell suspension was mixed in equal amounts so that the final concentration of DMSO was 10%. After equilibrating at 4°C for 20 minutes, the temperature was controlled with a temperature pro...

Claims

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Application Information

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IPC IPC(8): A01N1/02
Inventor 胡军祥姜玉新
Owner 胡军祥
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