Interferential RNA retrovirus and its preparation and use

A technology for retroviruses and uses, which is applied in the field of interfering RNA retroviruses and their preparation, can solve problems such as losses, and achieve effects of strong specificity, avoiding sequelae, and high technical content

Inactive Publication Date: 2005-08-17
田文志
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, due to various reasons, there is no treatment method that can completely cure leukemia and AIDS. This situation has brought great disasters to patients and their families, and has brought huge losses to the country.

Method used

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  • Interferential RNA retrovirus and its preparation and use
  • Interferential RNA retrovirus and its preparation and use
  • Interferential RNA retrovirus and its preparation and use

Examples

Experimental program
Comparison scheme
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Embodiment Construction

[0024] 1. Construction of retroviral vectors expressing interfering RNA

[0025] The DNA sequence of the mouse U6 promoter is loaded into a retroviral vector, and the interfering RNA of BCR / ABL (chronic myeloid leukemia) or CCR5 (HIV receptor) is installed downstream of the U6 promoter, which constitutes Retroviral vectors for expressing interfering RNA, such as figure 1 shown. The vector also contains the gene sequence for expressing green protein. Therefore, it can be used as an index to detect the efficiency of cell transfection or virus infection.

[0026] 2. Detect the interference activity of interfering RNA on the target gene

[0027] Using the method of transient transfection, the plasmid vector expressing OCILRP2 and the retroviral vector expressing interfering RNA were co-transfected into 293F cell line such as figure 2 As shown, after 48 hours, the cells were collected, and the expression of the target gene with red fluorescence was detected by flow cytometry a...

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Abstract

The interference RNA retrovirus is prepared through loading interference RNA to the pathogenetic gene of leukaemia or AIDS. The interference RNA retrovirus is used in infecting bone marrow cell or stem cell of target gene provider and the infected cell is transfused back to the body of the target gene provider. The reconstructed leukocyte has no pathogenetic gene, so that leukaemia or AIDS is cured. The present invention cures diseases through combining modern biological technology and bone marrow transplanting operation to deactivate pathogenetic gene. Owing to that the marrow for transplanting is from the patient, the present invention has no rejection.

Description

technical field [0001] The invention relates to an interfering RNA retrovirus and its preparation method and application. Background technique [0002] Both leukemia and AIDS are diseases of the blood cell system. The former is the continuous proliferation of immature white blood cells caused by genetic abnormalities. The patient loses normal resistance due to the lack of a normal number of mature white blood cells. At the same time, due to the large number of immature cells all over the body, the patient has repeated infections, ischemia, and finally dies of organ failure. AIDS is caused by the infection of human immune cells by the AIDS virus (HIV). Human immune cells have HIV receptors (such as CD4, CCR5, CXCR4). After binding to these receptors, HIV can invade immune cells and reproduce in large numbers in the cells, eventually destroying the cells. Patients suffer from severe immunodeficiency as a result and eventually die from severe infection or organ failure. So...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12N15/867
Inventor 田文志
Owner 田文志
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