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Method for preparing pumpkin polysaccharide

A technology of pumpkin polysaccharides and pumpkins, which is applied in the field of diabetes prevention and treatment products, and can solve problems such as troublesome eating and large dosage

Inactive Publication Date: 2005-06-08
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been reported that dehydrating, drying and grinding pumpkin to make pumpkin powder, which can control diabetes, but this method consumes a large amount and is troublesome to eat.

Method used

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  • Method for preparing pumpkin polysaccharide

Examples

Experimental program
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Effect test

Embodiment 1

[0012] Pumpkin powder is extracted with hot water, the extraction temperature is 50°C, the extraction time is 4 hours, the ratio of material to water is 1:8, after extraction, it is filtered, the filtrate is recovered, the filter residue is extracted with 95% ethanol for 48 hours, and the filtrate is collected, and the two filtrates are combined. To obtain the pumpkin primary extract, use 95% ethanol to precipitate the pumpkin primary extract at a ratio of 5:1 between ethanol and pumpkin primary extract, centrifuge to take the precipitate, wash it with absolute ethanol and petroleum ether in sequence, and then add 1 times the volume of 10 % trichloroacetic acid, stirred gently at room temperature and put it in the refrigerator overnight, centrifuged, discarded the precipitate, concentrated under reduced pressure, put it into a dialysis bag for dialysis against water for 1 day, double distilled water for 2 days, and took out and dried pumpkin polysaccharide.

Embodiment 2

[0014] Pumpkin powder is extracted with hot water, the extraction temperature is 80°C, the extraction time is 2 hours, the ratio of material to water is 1:10, after extraction, it is filtered, the filtrate is recovered, the filter residue is extracted with 95% ethanol for 30 hours, and the filtrate is collected, and the two filtrates are combined. To obtain the primary extract of pumpkin, use 95% ethanol to precipitate the primary extract of pumpkin according to the ratio of ethanol to the primary extract of pumpkin at a ratio of 3:1, centrifuge to take the precipitate, wash it successively with absolute ethanol and petroleum ether, and then add 1 times the volume of 10 % trichloroacetic acid, stirred gently at room temperature and put it in the refrigerator overnight, centrifuged, discarded the precipitate, concentrated under reduced pressure, put it into a dialysis bag for dialysis against water for 1 day, double distilled water for 2 days, and took out and dried pumpkin polys...

Embodiment 3

[0016] Pumpkin powder is extracted with hot water, the extraction temperature is 40°C, the extraction time is 5 hours, the ratio of material to water is 1:7, after extraction, it is filtered, the filtrate is recovered, the filter residue is extracted with 95% ethanol for 45 hours, and the filtrate is collected, and the two filtrates are combined. Obtain the primary extract of pumpkin, use 95% ethanol to precipitate the primary extract of pumpkin according to the ratio of ethanol to the primary extract of pumpkin 4:1, centrifuge to take the precipitate, wash it with absolute ethanol and petroleum ether in sequence, and then add 1 times the volume of 10 % trichloroacetic acid, stirred gently at room temperature and put it in the refrigerator overnight, centrifuged, discarded the precipitate, concentrated under reduced pressure, put it into a dialysis bag for dialysis against water for 1 day, double distilled water for 2 days, and took out and dried pumpkin polysaccharide.

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Abstract

A process for preparing pumpkin polysaccharose includes such steps as preparing pumpkin powder, extracting in hot water, filering, depositing in alcohol, washing deposit, adding trichloroacetic acid, laying aside in refrigerator, centrifugal separating, dialysis and drying.

Description

technical field [0001] The invention relates to a product for preventing and treating diabetes. Background technique [0002] Pumpkin, scientific name Cucurbita moschata Duch, alias pumpkin, rice melon, etc., is an annual vine herb with white spots on the leaves and pentagonal fruit stalks in Cucurbitaceae and Cucurbita. The nutritional value of pumpkin is very high. In ancient my country, there were records of the therapeutic and health effects of pumpkin. The history book of traditional Chinese medicine "Southern Yunnan Materia Medica" records: "Pumpkin is warm in nature, sweet and non-toxic, enters the spleen and stomach, can moisten the lungs and replenish qi, reduce phlegm and discharge pus, drive insects and detoxify, and treat cough, asthma, lung abscess, Constipation and other diseases", and it is believed that pumpkin can "run across the meridians and facilitate urination". In "Compendium of Materia Medica", Li Mingzhen put pumpkin and Gano...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715C08B37/00
Inventor 张拥军王正郁郑龙华
Owner CHINA JILIANG UNIV
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