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Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour

A sphingomonas, anthraquinone dye technology, applied in the fields of bioengineering and environmental engineering, can solve the problem of no evolution position, and achieve the effects of fast degradation speed, enhanced processing ability, and strong decolorization ability

Inactive Publication Date: 2005-05-25
DALIAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of traditional bacterial classification methods, this genus has not had an independent evolutionary position in systematic taxonomy.

Method used

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  • Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour
  • Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour
  • Sphingol monospore bacterial strain and its application in anthraquinone dye waste water decolour

Examples

Experimental program
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Effect test

Embodiment 1

[0042] The screening steps of the Sphingomonas bacterial strain provided by the invention:

[0043] The aerobic activated sludge samples were collected from the sewage outlet of the bromine production workshop of Zhaoyuan General Chemical Plant in Shandong Province, and used as a source of bacteria for domestication and enrichment. That is to say, the collected sludge is made into a suspension, and the supernatant is added to the activation enrichment medium for enrichment. The composition of the medium (g / l): beef extract 5, peptone 10, NaCl 5, wherein the medium is at 121 ℃, autoclaved for 20 minutes before use. Then transfer to the substratum with bromine as the only carbon source for acclimatization, substratum composition (g / l): (NH 4 ) 2 SO 4 2. KH 2 PO 4 1.3, Na 2 HPO 4 2. Trace elements 1ml / l, of which the composition of trace elements (g / l): (MnSO 4 ·H 2 O 39.9, ZnSO 4 9H 2 O 68.55, (NH 4 ) 6 Mo 7 o 24 4H 2 O 34.7. Acclimatization adopts the domest...

Embodiment 2

[0058] The application of the present invention to the decolorization research of bromic acid in nutrient-enriched medium, its steps are as follows:

[0059] [1] Add 50ml of LB medium to a 250ml Erlenmeyer flask, then add bromine to make the final concentration 100mg / l, and sterilize for later use.

[0060] [2] Add the cell liquid culture of the sphingomonas strain prepared in Example 1 or the dormant cells of the sphingomonas strain prepared in the embodiment into the Erlenmeyer flask of the above [1] , 30°C, 150r / min, aerobic culture. Samples were taken every 12 hours. figure 1 is the growth of Sphingomonas strains in the enriched medium and the degradation of bromine; it can be seen from the figure that the degradation of bromine occurs in the early stage of the growth of the strain instead of the logarithmic phase; and it can be seen that the bacteria A small amount of body growth is enough to degrade bromine, and the decolorization rate of bromine is as high as 100% 14 ...

Embodiment 3

[0062] Application of the present invention in decolorizing research on bromic acid in medium with bromic acid as the sole carbon and nitrogen source:

[0063] The LB medium in Example 1 was replaced with the basal medium with bromine as the sole carbon and nitrogen source, and other steps were the same as in Example 6. image 3 is the degradation situation of growing cells to different concentrations of bromine; image 3 It can be seen that the strain of Sphingomonas has a very strong decolorization ability to bromine, and can tolerate the concentration of bromine as high as 1000ppm.

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Abstract

A sphingomonas xenophaga QYY (CGMCC No.1172) is disclosed, which features that the bromamino acid as the intermediate of anthraquinone dye is the unique carbon and nitrogen sources for its growth, so its cells can destroy the anthraquinone rings. As a result, it has strong decolor capacity and can be used to decolor the sewage of anthraquinone dye.

Description

technical field [0001] The invention belongs to the technical fields of biological engineering and environmental engineering. It relates to the strain of Sphingomonas and its application in the decolorization of anthraquinone dye wastewater. Background technique [0002] Inefficient dye production processes result in large amounts of unutilized dye directly entering water bodies. The color of dye wastewater directly indicates that the water body is polluted. Therefore, the removal of dyes in water is a hotspot in the field of environmental research. Compared with azo dyes, anthraquinone dyes have limited biodegradation due to their structural stability. Therefore, so far, the work on the biodegradation of anthraquinone dyes is still very limited. Itoh ketal et al. studied the biodegradation of anthraquinone dye C.I.Risperse Red 15 by Bacillus Subtilis. Jiang and Bishop reported the aerobic degradation of acid anthraquinone dyes using a rotating membrane bioreactor. G.M...

Claims

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Application Information

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IPC IPC(8): C02F1/58C02F3/34C12N1/20
Inventor 周集体曲媛媛王竞吕红邢林林夏元化张劲松项学敏金若菲
Owner DALIAN UNIV OF TECH
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