Applied culture technology for salt tolerant rhodospirillum rubrum

Abstract

The invention involves a kind to be good lowly, using the sea water cultivation effect bears the salty rapana thomasiana microbial inoculum using the microbial inoculum raise expense the application raise technology. Bears the salty red spirillum the selective breeding for to concentrate, the plate separation, the strain function selective breeding from the sea water, bears the salty red spirillum function strain. Using raise technology including culture medium optimization and application raise craft condition improvement. The culture medium optimization is from carbon source, nitrogen source, ingredient and so on inorganic salt and growth factor carries on the optimization, obtains reduces 47% culture medium ingredient the application formula. Uses the application formula postpositioned using the microbial inoculum raise, the vaccination under the white plastic warm awning the sunlight raise. The invention bears the salty rapana thomasiana strain to be possible to grow under the 0.1~~4%NaCl density, in the fresh water, the sea water cultivation may receive the good application effect. It greatly reduced the photosynthesis bacilliculture cost and the equipment using the raise technology invests. Bears the salty rapana thomasiana microbial inoculum the application raise to issue each milliliter hundred million cells in the above condition the use density to request.

Description

Technical field: [0001] The invention relates to the selection and breeding of salt-tolerant Rhodospirillum, the screening of application-type medium formula and the method of applying culture bacteria agent. To be precise, it provides a photosynthetic bacterial agent that can be used in freshwater and seawater aquaculture, and provides an applied formula that can greatly reduce the cost of the medium, and reduce equipment, energy consumption, and cultivation operations. Low-cost applied culture technology with simple process. Background technique: [0002] Photosynthetic bacteria are microbial resources that are devoted to development at home and abroad. Among the photosynthetic bacteria groups, Rhodospiraceae photosynthetic bacteria can grow heterotrophically under aerobic and anaerobic conditions, so they have received more and more attention in the application of wastewater biological treatment and aquaculture. At present, most of the micro-ecological agents for aquacu...

AI Technical Summary

Problems solved by technology

[0005] The present invention aims to fundamentally solve the problem of poor salt tolerance of Rhodospirillum agent and poor effect of application of seawater aquaculture, as well as high cultivation cost of application of agent, and provides a kind of antisalt agent that has good salt tolerance and can be used in both fresh water and seawater aquaculture. Applied culture technology of salt-tolerant rhodospirillum agent with good efficacy and low cost of applied medium formula