Process for preparing tissue chips and apparatus thereof

A tissue chip and preparation device technology, applied in molecular biology research and new preparation fields, can solve the problems of difficulty in making high-density, high-quality tissue chips, high process cost, inconvenient positioning, etc., and is conducive to popularization. The effect of promoting, improving chip quality and reducing process cost

Inactive Publication Date: 2005-03-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are existing patent documents reporting methods for preparing tissue chips, such as the Chinese invention patent application number: 02139474.1, which records a method for preparing tissue chips, which needs to be melted in a relatively long period of time through a constant temperature water bath with a temperature control switch. The temperature of paraffin is slightly higher than its melting point within the range of 5°C to 10°C, and a negative pressure pump connected to the negative pressure box below the embedding tank is required to form a certain negative pressure at the bottom of the embedding tank, and the structure is relatively complicated. The process cost is high, and it is still repeated spotting one by one
China Invention Patent Application No.: 02113230.5, which records the use of semi-melted paraffin to make tissue chips. This method needs to maintain the semi-melted state of paraffin for a long time, which is inconvenient for positioning during the sampling process, and it is difficult to produce high-density and high-quality tissues. chip
European patent WO 2004 / 000992 and U.S. patent PAT.NO.: 6,103,518 have recorded the improvement of the method focusing on the selection of typical tissue lesion sites, and chip production is still a traditional process, and the production cost is relatively high

Method used

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  • Process for preparing tissue chips and apparatus thereof
  • Process for preparing tissue chips and apparatus thereof
  • Process for preparing tissue chips and apparatus thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] see Figure 1-Figure 5 , the tissue chip preparation device is mainly composed of a sampling instrument I and a preparation instrument II, and the sampling instrument I mainly includes a control pressure rod 1, an ejector rod 2, a guide slider 3, a fixed nut 4, a sampling needle 5 with a needle core 11, a return Position clip spring 6, positioning bolt 7, positioning block 8, guide slide bar 9, base 10, guide slide bar 9 is set on base 10, return clip spring 6 is connected with guide slide block 3, and guide slide bar 3 is provided with The fixed nut 4, the sampling needle 5 is fixed with the guide slider 3 through the fixed nut 4, the guide slider 3 is connected with one end of the ejector rod 2, the other end of the ejector rod 2 is connected with the control rod 1, and the control rod 1 is connected with the The top of the guide slider 9 is connected flexibly. When the control lever 1 is pressed down, the guide slider 3 is driven down by the ejector rod 2, and the sa...

Embodiment 2

[0023] refer to Figure 1-Figure 2 After the sample wax block is marked according to conventional techniques, the marked sample wax block is placed on the base 10 of the sampling instrument 1, the position of the positioning block 8 is fixed on the guide slide bar 9 by the positioning bolt 7, and a sampling needle 5 Fix the guide block 3 through the fixed nut 4, press down the control lever 1, and move the guide block 3 down through the ejector rod 2, so that the front end of the sampling needle 5 enters the sample wax block, and the needle core 11 will be taken The tissue sample is jacked up, and the height of the needle core 11 is equal to the height of the sample column to be taken. Release the control lever 1, and use the return compression spring 6 to lift the sampling needle 5 to complete a sampling. Figure 5 Arrange the sampled sampling needle 5 together with the lifted needle core 11 in the chip array arrangement plate 15 according to the bit sequence of the chip desi...

Embodiment 3

[0025] According to different test purposes and chip designs, chip arrays with different sampling diameters and different densities can be selected. The system can be equipped with sampling needles of various specifications (commonly used specifications are 0.6mm, 1.0mm, and 1.5mm), and they are matched with array layout plates of corresponding apertures.

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Abstract

It is organization chip process method and its apparatus which comprises sample device 1 and process device 11 and in detail comprises control pressure bar1, guiding module 3, fixed screw 4, sample needle 5 of needle chip 11, reset pressure spring 6, position bolt 7, guiding bar 9, control bolt 12, needle chip control panel 14, array distribution board 15, L-shape board 16 and guiding bar 17. It is to centrally sample through sample device and then to distribute a group of sample needles 5 orderly on the array distribution board 15, and to inject olefin into all the organic samples through pushdown needle chip control board 14 and it is finished when the olefin is solidified. This apparatus in this invention is in no need of olefin block process and stiletto procedure and accuracy position system.

Description

technical field [0001] The invention belongs to biotechnology, relates to tissue chip technology, in particular to a new preparation method of tissue chip and its device, which is suitable for molecular biology research. Background technique [0002] Biochip (biochip) is a high-throughput and high-tech technology developed in the mid-1990s for molecular biology research. It is another biotechnology revolution with great influence after large-scale integrated circuits ( Watson S J, et al., Biol Psychiatry, 1999). According to different biological materials, biochips can be divided into many kinds, such as gene chips, protein chips, cell chips, microorganism chips, tissue chips, etc. In 1998, Kenonen J et al. (Frantz G D, et al., J Pathol, 2001) first reported tissue chip technology, also called tissue microarray (tissue microarray, TMA). Applying this technology, in situ molecular biology research such as immunohistochemistry and in situ hybridization can be performed on do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/02G01N1/28G01N33/48
Inventor 孟潘庆周桂英郑树罗建明董琪
Owner ZHEJIANG UNIV
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