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Anti-human phylaxin synthetic polypeptide antibody and its preparation method

A technology for synthesizing polypeptides and resistin, applied in the direction of anti-animal/human immunoglobulin, etc., to achieve the effect of good specificity, strong affinity and high titer

Inactive Publication Date: 2005-03-16
INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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Problems solved by technology

[0006] The present invention aims to solve the problem that the laboratory is used in immunoprecipitation and Western blot tests to establish experimental biological products required for in vitro immune analysis, and provides an anti-human resistin synthetic polypeptide antibody and its preparation method

Method used

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Embodiment Construction

[0014] 1. Synthesis of resistin antigenic peptide: using Antheprot protein analysis software to analyze the hydrophilicity, antigenic type and homology of human resistin amino acid sequence (1-108). A peptide sequence containing 13 amino acids at positions 22-34 was selected as the antigenic peptide, and the crude product was obtained after synthesis from the fixed carboxyl group to the amino terminal on a 433A automatic peptide synthesizer. After being dissolved in 30% acetonitrile, it was analyzed by high pressure liquid chromatography (HPLC), and the area of ​​the main peak was calculated and collected. The synthesized peptide was purified after refrigerated vacuum drying, and identified by mass spectrometry.

[0015] 2. Coupling of the synthetic polypeptide and the carrier: the carrier protein is selected from keyhole limpet hemocyanin (KLH, keyhole limpet hemocyanin), and the KLH is linked with the bifunctional reagent maleamide benzoic acid-N-succinate (MBS). Coupling wi...

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Abstract

The invention discloses an anti-human phylaxin synthetic polypeptide antibody and its preparation method which consists of, synthesizing polypeptides using one segment of the resistin amino acid sequence, using its amino acid sequence CSMEEAINERIQE as semiantigen, preparing artificial immunity antigen through connection with protein carriers, immunizing the animals so as to effectively induce organism immune response, thus obtaining the antibodies.

Description

technical field [0001] The invention relates to biological products for in vitro tests characterized by antibodies, in particular to an anti-human resistin synthetic polypeptide antibody and a preparation method thereof. Background technique [0002] Resistin (RST) is a hormone protein specifically expressed and secreted by white adipocytes discovered in 2001. Due to the high price of commercially available recombinant human resistin, the cost of preparing antibodies is too high, which is both risky and uneconomical. [0003] ab1129: http: / / www.abcam.com / ? datasheet=1129 "Resistin antibody (ab1129) data sheet" supplier NovuS-Biologicals (NB200-202) provides rabbit anti-resistin polyclonal antibody, its immunogen synthetic peptide GSWDVRAETTCH, corresponding to 79-90 of the amino acid sequence of human resistin bit. [0004] ab1130: http: / / www.abcam.com / ? datasheet=1130 "Resistin antibody (ab1129) data sheet" supplier Novus-Biologicals (NB200-202) provides rabbit anti-res...

Claims

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Application Information

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IPC IPC(8): C07K16/18
Inventor 赵明辉韩佩珍张春明王胜芬王德芝王彦刘庆芬陈冀莹
Owner INST OF RADIATION MEDICINE CHINESE ACADEMY OF MEDICAL SCI
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